PMID- 20075416
OWN - NLM
STAT- MEDLINE
DCOM- 20110104
LR  - 20211020
IS  - 1939-4640 (Electronic)
IS  - 0196-3635 (Linking)
VI  - 31
IP  - 4
DP  - 2010 Jul-Aug
TI  - Glucocorticoid suppresses steroidogenesis in rat progenitor Leydig cells.
PG  - 365-71
LID - 10.2164/jandrol.109.009019 [doi]
AB  - Glucocorticoid (GC) inhibits testosterone production in adult Leydig cells by the 
      glucocorticoid receptor (GR). However, whether GC affects the development of 
      Leydig cells is unclear. The goal of the present study is to investigate the 
      effects of GC on steroidogenesis of rat progenitor Leydig cells (PLCs) in vitro. 
      Dexamethasone (DEX) inhibited androsterone (AO) production in PLCs. The GR 
      antagonist RU38486 reversed the DEX-induced inhibition of AO, whereas the 
      mineralocorticoid receptor antagonist RU28318 did not. RU38486 also reversed 
      DEX-induced reductions in steady-state mRNA levels of steroidogenic acute 
      regulatory protein (Star) and 3beta-hydroxysteroid dehydrogenase 1 (Hsd3b1). 
      Steroidogenic acute regulatory protein (StAR) protein expression and 
      3beta-hydroxysteroid dehydrogenase (3betaHSD) enzyme activity were affected similarly. 
      These results show that GCs inhibit steroidogenesis of PLCs by suppression of 
      StAR and 3betaHSD via a GR-mediated mechanism.
FAU - Xiao, Ye-Chen
AU  - Xiao YC
AD  - Population Council, New York, NY 10065, USA.
FAU - Huang, Ya-Dong
AU  - Huang YD
FAU - Hardy, Dianne O
AU  - Hardy DO
FAU - Li, Xiao-Kun
AU  - Li XK
FAU - Ge, Ren-Shan
AU  - Ge RS
LA  - eng
GR  - R01 HD050570/HD/NICHD NIH HHS/United States
GR  - R01 HD050570-03/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100114
PL  - United States
TA  - J Androl
JT  - Journal of andrology
JID - 8106453
RN  - 0 (Androgens)
RN  - 0 (Glucocorticoids)
RN  - 0 (Mineralocorticoid Receptor Antagonists)
RN  - 0 (Phosphoproteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (steroidogenic acute regulatory protein)
RN  - 27O7W4T232 (Spironolactone)
RN  - 320T6RNW1F (Mifepristone)
RN  - 76676-34-1 (RU 28318)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.1.- (3-Hydroxysteroid Dehydrogenases)
SB  - IM
MH  - 3-Hydroxysteroid Dehydrogenases/metabolism
MH  - Androgens/*biosynthesis
MH  - Animals
MH  - Dexamethasone/*pharmacology
MH  - Gene Expression/drug effects
MH  - Glucocorticoids/*pharmacology
MH  - Leydig Cells/*drug effects
MH  - Male
MH  - Mifepristone/pharmacology
MH  - Mineralocorticoid Receptor Antagonists/pharmacology
MH  - Phosphoproteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Glucocorticoid/*antagonists & inhibitors
MH  - Spironolactone/analogs & derivatives/pharmacology
MH  - Stem Cells/drug effects
EDAT- 2010/01/16 06:00
MHDA- 2011/01/05 06:00
CRDT- 2010/01/16 06:00
PHST- 2010/01/16 06:00 [entrez]
PHST- 2010/01/16 06:00 [pubmed]
PHST- 2011/01/05 06:00 [medline]
AID - jandrol.109.009019 [pii]
AID - 10.2164/jandrol.109.009019 [doi]
PST - ppublish
SO  - J Androl. 2010 Jul-Aug;31(4):365-71. doi: 10.2164/jandrol.109.009019. Epub 2010 
      Jan 14.