PMID- 20079209
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 48
IP  - 9
DP  - 2009 Sep
TI  - [The comparison of three cytogenetic methods for detecting multiple myeloma].
PG  - 737-40
AB  - OBJECTIVE: To explore the value of conventional cytogenetic technique (CC), 
      modified cell culture (long term culture and increasing the final concentration 
      of colcemid) and fluorescence in situ hybridization (FISH) in detection of 
      chromosomal and genomic aberrations of multiple myeloma(MM). METHODS: RHG banding 
      was used to evaluate the efficiency of modified culture method on abnormal 
      karyotype detection in 21 MM patients. The probes 1q21, 13q14 (RB1), 14q32 
      (IGHC/IGHV gene) were used to perform FISH in the detection of chromosomal and 
      genomic aberrations of MM. RESULTS: Abnormal chromosomes were detected in 4 cases 
      (19.1%) of the 21 MM patients with CC. After modified cell culture, abnormal 
      karyotype was detected in 6 cases (28.6%). Abnormal chromosomes were detected in 
      12 of 18 cases (66.7%) using FISH. Panel FISH disclosed 1q21 amplification in 4 
      (22.2%), del(13q) abnormality in 5 (27.8%), 14q32 rearrangement in 8 (44.4%). 
      CONCLUSION: FISH can significantly improve the detection rate of chromosomal 
      aberrations in MM.
FAU - Ren, Juan
AU  - Ren J
AD  - Department of Hematology, the Second Hospital of Shanxi Medical University, 
      Taiyuan 030001, China.
FAU - Yang, Lin-hua
AU  - Yang LH
FAU - Li, Guo-xia
AU  - Li GX
FAU - Fan, Jian-ling
AU  - Fan JL
FAU - Hou, Jian
AU  - Hou J
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
SB  - IM
MH  - Aged
MH  - Chromosome Aberrations
MH  - Cytogenetics/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - Tumor Cells, Cultured
EDAT- 2010/01/19 06:00
MHDA- 2010/05/07 06:00
CRDT- 2010/01/19 06:00
PHST- 2010/01/19 06:00 [entrez]
PHST- 2010/01/19 06:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2009 Sep;48(9):737-40.