PMID- 20079231
OWN - NLM
STAT- MEDLINE
DCOM- 20100506
LR  - 20140226
IS  - 0578-1426 (Print)
IS  - 0578-1426 (Linking)
VI  - 48
IP  - 10
DP  - 2009 Oct
TI  - [A preliminary study on the effects of feto-maternal microchimerism in activated 
      human leukocyte antigen haploidentical mobilized peripheral blood stem cells on 
      treatment of solid tumors].
PG  - 857-61
AB  - OBJECTIVE: To study the effect of feto-maternal microchimerism in the treatment 
      of activated human leukocyte antigen (HLA) haploidentical mobilized peripheral 
      blood cells against solid tumors. METHODS: Genomic DNA samples of 25 pairs of HLA 
      haploidentical donors and recipients were extracted. The donor-derived HLA-DRB 
      loci were detected with nested PCR-sequence specific primer (SSP) typing. The 
      mixed lymphocyte proliferation action between the patients and respective donors, 
      the engraftment of donor's cells and the serum levels of Th1/Th2 type of 
      cytokines were measured with MTT, FISH and ELISA method respectively. The 
      survival time of patients with or without feto-maternal microchimerism were 
      compared as well. RESULTS: Using nested PCR-SSP typing, the positive rates of 
      feto-maternal microchimerism in the 25 pairs of HLA haploidentical donors and 
      recipients were 40% in the maternal/children pairs and 0 in the paternal/children 
      pairs. The chimerism positive patients showed less proliferation activity when 
      cocultured with respective donors as compared with unrelated ones (P = 0.03). 
      Only one chimerism positive patient experienced the engraft of donor's cell 3 
      months after treatment as the donor derived XX chromosome was identified with 
      FISH. When the data of chimerism positive patients were deleted, the serum levels 
      of IFNgamma 1 month after treatment dropped dramatically from 171.4 (26.3 
      approximately 258.4) ng/L to 29.4 (1.2 approximately 39.9) ng/L. The survival 
      time in chimerism positive patients of the maternal/children pairs was 
      significantly longer than that in chimerism negative patients, which was (31.2 
      +/- 4.3) months and (11.1 +/- 3.3) months, respectively (P = 0.036). CONCLUSION: 
      Feto-maternal microchimerism might induce anergy in the HLA haploidentical 
      donors, favor the engraftment of donor's progenitors and maintenance of positive 
      microenvironment and prolong the survival time.
FAU - Cao, Shui
AU  - Cao S
AD  - Department of Biological Therapy, Tianjin Medical University Cancer Institute and 
      Hospital, Tianjin 300060, China.
FAU - Yu, Jin-pu
AU  - Yu JP
FAU - Li, Hui
AU  - Li H
FAU - An, Xiu-mei
AU  - An XM
FAU - Xin, Ning
AU  - Xin N
FAU - Ren, Xiu-bao
AU  - Ren XB
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Nei Ke Za Zhi
JT  - Zhonghua nei ke za zhi
JID - 16210490R
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chimerism
MH  - Female
MH  - Graft Rejection/prevention & control
MH  - HLA Antigens/*genetics/immunology
MH  - Haploidy
MH  - Histocompatibility Testing
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/*genetics/immunology/therapy
MH  - *Peripheral Blood Stem Cell Transplantation
MH  - Transplantation Conditioning/*methods
MH  - Transplantation, Homologous
EDAT- 2010/01/19 06:00
MHDA- 2010/05/07 06:00
CRDT- 2010/01/19 06:00
PHST- 2010/01/19 06:00 [entrez]
PHST- 2010/01/19 06:00 [pubmed]
PHST- 2010/05/07 06:00 [medline]
PST - ppublish
SO  - Zhonghua Nei Ke Za Zhi. 2009 Oct;48(10):857-61.