PMID- 20079253
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20100118
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 44
IP  - 9
DP  - 2009 Sep
TI  - [Hypoxia regulates osteogenic differentiation of human periodontal ligament stem 
      cells].
PG  - 543-7
AB  - OBJECTIVE: To investigate the effect of hypoxia on isolated human periodontal 
      ligament stem cells (hPDLSC) in vitro. METHODS: hPDLSC were exposed to normoxia 
      (20% O(2)) and hypoxia (1.5% - 2% O(2)). Total cellular RNA and protein were 
      collected on day 1, 2, 4 and 6 after culture. Western analysis and 
      semi-quantitative RT-PCR were used to analyze osteogenic differentiation of 
      hPDLSC, including alkaline phosphatase (ALP), osteocalcin (OCN), secreted protein 
      acidic and rich in cysteine (SPARC) and bone morphogenetic protein-2 (BMP-2). 
      RESULTS: In the first two days hypoxia slightly increased the growth of hPDLSC [A 
      value was 0.697(hypoxia) vs 0.617 (nomoxia)] and after the third day hypoxia 
      dramatically decreased the growth of the hPDLSC [A value was 0.870(hypoxia) vs 
      1.242 (nomoxia)]. Up to 90% reduction of ALP activity was observed in hPDLSC 
      after 5 days of hypoxia [A value was 0.004(hypoxia) vs 0.049(nomoxia)]. Hypoxia 
      decreased SPARC expression at protein level and down-regulated ALP, OCN and BMP-2 
      expression at mRNA level in comparison with nomoxia. CONCLUSIONS: Hypoxia 
      inhibited proliferation of hPDLSC and down-regulated BMP-2 mRNA expression, then 
      down-regulated their target genes such as ALP, OCN, and SPARC, thus inhibiting 
      critical steps in osteogenic differentiation of hPDLSC.
FAU - Hou, Jian-xia
AU  - Hou JX
AD  - Department of Periodontology, Peking University School and Hospital of 
      Stomatology, Beijing 100081, China. jxhou@163.com
FAU - Loomer, Peter M
AU  - Loomer PM
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
SB  - IM
MH  - Bone Morphogenetic Protein 2/metabolism
MH  - *Cell Differentiation
MH  - Cell Hypoxia
MH  - Cells, Cultured
MH  - Humans
MH  - Periodontal Ligament/*cytology/metabolism
MH  - Stem Cells/*cytology/metabolism
EDAT- 2010/01/19 06:00
MHDA- 2015/04/10 06:00
CRDT- 2010/01/19 06:00
PHST- 2010/01/19 06:00 [entrez]
PHST- 2010/01/19 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2009 Sep;44(9):543-7.