PMID- 20080316
OWN - NLM
STAT- MEDLINE
DCOM- 20100909
LR  - 20220321
IS  - 1618-1328 (Electronic)
IS  - 0176-1617 (Linking)
VI  - 167
IP  - 8
DP  - 2010 May 15
TI  - Transgenic expression of the Trichoderma harzianum hsp70 gene increases 
      Arabidopsis resistance to heat and other abiotic stresses.
PG  - 659-65
LID - 10.1016/j.jplph.2009.11.012 [doi]
AB  - The ability of some Trichoderma strains, a biological control agent, to overcome 
      extreme environmental conditions has previously been reported and related to 
      heat-shock proteins (HSPs). These proteins are induced environmentally and are 
      involved in important processes, acting as molecular chaperones in all organisms. 
      In a previous study, we demonstrated, by overexpression, that the Trichoderma 
      harzianum hsp70 gene conferred tolerance to heat and other abiotic stresses to 
      this fungus. In this work, we investigate the function of the T. harzianum T34 
      hsp70 gene in Arabidopsis thaliana. We analyze transgenic plant responses under 
      adverse environmental conditions and the expression levels of a set of seven 
      stress genes, using quantitative RT-PCR. As expected, transgenic plants 
      expressing the T. harzianum hsp70 gene exhibited enhanced tolerance to heat 
      stress. In addition, they did not show growth inhibition and, after heat 
      pre-treatment, transgenic seedlings were more tolerant to osmotic, salt and 
      oxidative stresses with respect to the wild-type behavior. Transgenic lines also 
      had increased transcript levels of the Na(+)/H(+) exchanger 1 (SOS1) and 
      ascorbate peroxidase 1 (APX1) genes, involved in salt and oxidative stress 
      responses, respectively. However, the heat-shock factor (HSF) and four HSP genes 
      tested were down-regulated in 35S:hsp70 plants. Overall, our results indicate 
      that hsp70 confers tolerance to heat and other abiotic stresses and that the 
      fungal HSP70 protein acts as a negative regulator of the HSF transcriptional 
      activity in Arabidopsis.
CI  - (c) 2009 Elsevier GmbH. All rights reserved.
FAU - Montero-Barrientos, Marta
AU  - Montero-Barrientos M
AD  - Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Departamento de 
      Microbiologia y Genetica, Universidad de Salamanca, Rio Duero 12, 37185 
      Villamayor, Salamanca, Spain.
FAU - Hermosa, Rosa
AU  - Hermosa R
FAU - Cardoza, Rosa E
AU  - Cardoza RE
FAU - Gutierrez, Santiago
AU  - Gutierrez S
FAU - Nicolas, Carlos
AU  - Nicolas C
FAU - Monte, Enrique
AU  - Monte E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100118
PL  - Germany
TA  - J Plant Physiol
JT  - Journal of plant physiology
JID - 9882059
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (HSP70 Heat-Shock Proteins)
SB  - IM
MH  - Adaptation, Physiological/genetics
MH  - Arabidopsis/*genetics/*physiology
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Down-Regulation/genetics
MH  - Gene Expression Regulation, Plant
MH  - Germination
MH  - HSP70 Heat-Shock Proteins/*genetics/metabolism
MH  - Heat-Shock Response/genetics
MH  - *Hot Temperature
MH  - Hypocotyl/growth & development
MH  - Osmotic Pressure
MH  - Oxidative Stress/genetics
MH  - Phenotype
MH  - Plants, Genetically Modified
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Stress, Physiological
MH  - Trichoderma/*genetics
EDAT- 2010/01/19 06:00
MHDA- 2010/09/10 06:00
CRDT- 2010/01/19 06:00
PHST- 2009/09/23 00:00 [received]
PHST- 2009/11/12 00:00 [revised]
PHST- 2009/11/15 00:00 [accepted]
PHST- 2010/01/19 06:00 [entrez]
PHST- 2010/01/19 06:00 [pubmed]
PHST- 2010/09/10 06:00 [medline]
AID - S0176-1617(09)00515-X [pii]
AID - 10.1016/j.jplph.2009.11.012 [doi]
PST - ppublish
SO  - J Plant Physiol. 2010 May 15;167(8):659-65. doi: 10.1016/j.jplph.2009.11.012. 
      Epub 2010 Jan 18.