PMID- 20081153
OWN - NLM
STAT- MEDLINE
DCOM- 20101104
LR  - 20211020
IS  - 1535-9484 (Electronic)
IS  - 1535-9476 (Print)
IS  - 1535-9476 (Linking)
VI  - 9
IP  - 7
DP  - 2010 Jul
TI  - Multiple, non-conserved, internal viral ligands naturally presented by HLA-B27 in 
      human respiratory syncytial virus-infected cells.
PG  - 1533-9
LID - 10.1074/mcp.M900508-MCP200 [doi]
AB  - Cytotoxic T lymphocyte (CTL)-mediated death of virus-infected cells requires 
      prior recognition of short viral peptide antigens that are presented by human 
      leukocyte antigen (HLA) class I molecules on the surface of infected cells. The 
      CTL response is critical for the clearance of human respiratory syncytial virus 
      (HRSV) infection. Using mass spectrometry analysis of complex HLA-bound peptide 
      pools isolated from large amounts of HRSV-infected cells, we identified nine 
      naturally processed HLA-B27 ligands. The isolated peptides are derived from six 
      internal, not envelope, proteins of the infective virus. The sequences of most of 
      these ligands are not conserved between different HRSV strains, suggesting a 
      mechanism to explain recurrent infection with virus of different HRSV antigenic 
      subgroups. In addition, these nine ligands represent a significant fraction of 
      the proteome of this virus, which is monitored by the same HLA class I allele. 
      These data have implications for vaccine development as well as for analysis of 
      the CTL response.
FAU - Infantes, Susana
AU  - Infantes S
AD  - Unidad de Protemica, Centro Nacional de Microbiologia, Instituto de Salud Carlos 
      III, 28220 Majadahonda, Madrid, Spain.
FAU - Lorente, Elena
AU  - Lorente E
FAU - Barnea, Eilon
AU  - Barnea E
FAU - Beer, Ilan
AU  - Beer I
FAU - Cragnolini, Juan Jose
AU  - Cragnolini JJ
FAU - Garcia, Ruth
AU  - Garcia R
FAU - Lasala, Fatima
AU  - Lasala F
FAU - Jimenez, Mercedes
AU  - Jimenez M
FAU - Admon, Arie
AU  - Admon A
FAU - Lopez, Daniel
AU  - Lopez D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100115
PL  - United States
TA  - Mol Cell Proteomics
JT  - Molecular & cellular proteomics : MCP
JID - 101125647
RN  - 0 (Antigens, Viral)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, Viral/chemistry/genetics/*immunology
MH  - Histocompatibility Antigens Class I/genetics/immunology
MH  - Humans
MH  - *Ligands
MH  - Molecular Sequence Data
MH  - Peptides/chemistry/genetics/immunology
MH  - Respiratory Syncytial Virus Infections/*immunology
MH  - Respiratory Syncytial Virus, Human/*immunology
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - T-Lymphocytes, Cytotoxic/immunology
PMC - PMC2938088
EDAT- 2010/01/19 06:00
MHDA- 2010/11/05 06:00
PMCR- 2011/07/01
CRDT- 2010/01/19 06:00
PHST- 2010/01/19 06:00 [entrez]
PHST- 2010/01/19 06:00 [pubmed]
PHST- 2010/11/05 06:00 [medline]
PHST- 2011/07/01 00:00 [pmc-release]
AID - S1535-9476(20)30944-0 [pii]
AID - M900508-MCP200 [pii]
AID - 10.1074/mcp.M900508-MCP200 [doi]
PST - ppublish
SO  - Mol Cell Proteomics. 2010 Jul;9(7):1533-9. doi: 10.1074/mcp.M900508-MCP200. Epub 
      2010 Jan 15.