PMID- 20081813
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Linking)
VI  - 23
IP  - 3
DP  - 2010 Mar
TI  - Classifying ambiguous melanocytic lesions with FISH and correlation with clinical 
      long-term follow up.
PG  - 413-9
LID - 10.1038/modpathol.2009.177 [doi]
AB  - Recently, initial studies describing the use of multicolor fluorescence in situ 
      hybridization (FISH) for classifying melanocytic skin lesions have been published 
      demonstrating a high sensitivity and specificity in discriminating melanomas from 
      nevi. However, the majority of these studies included neither histologically 
      ambiguous lesions nor a clinical long-term follow up. This study was undertaken 
      to validate a special multicolor FISH test in histologically ambiguous 
      melanocytic skin lesions with known clinical long-term follow up. FISH was scored 
      by three independent pathologists in a series of 22 melanocytic skin lesions, 
      including 12 ambiguous cases using four probes targeting chromosome 6p25, 
      centromere 6, 6q23, and 11q13. The FISH results were compared with array 
      comparative genomic hybridization data and correlated to the clinical long-term 
      follow up (mean: 65 months). Pair-wise comparison between the interpretations of 
      the observers showed a moderate to substantial agreement (kappa 0.47-0.61). 
      Comparing the FISH results with the clinical behavior reached an overall 
      sensitivity of 60% and a specificity of 50% (chi(2)=0.25; P=0.61) for later 
      development of metastases. Comparison of array comparative genomic hybridization 
      data with FISH analyses did not yield significant results but array comparative 
      genomic hybridization data demonstrated that melanocytic skin lesions with the 
      development of metastases showed significantly more chromosomal aberrations 
      (P<0.01) compared with melanocytic skin lesions without the development of 
      metastases. The FISH technique with its present composition of locus-specific 
      probes for RREB1/MYB and CCND1 did not achieve a clinically useful sensitivity 
      and specificity. However, a reassessment of the probes and better standardization 
      of the method may lead to a valuable diagnostic tool.
FAU - Gaiser, Timo
AU  - Gaiser T
AD  - Department of Neuropathology, Institute of Pathology, University of Heidelberg, 
      Germany. gaisert@mail.nih.gov
FAU - Kutzner, Heinz
AU  - Kutzner H
FAU - Palmedo, Gabriele
AU  - Palmedo G
FAU - Siegelin, Markus D
AU  - Siegelin MD
FAU - Wiesner, Thomas
AU  - Wiesner T
FAU - Bruckner, Thomas
AU  - Bruckner T
FAU - Hartschuh, Wolfgang
AU  - Hartschuh W
FAU - Enk, Alexander H
AU  - Enk AH
FAU - Becker, Maria R
AU  - Becker MR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20100115
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations
MH  - Comparative Genomic Hybridization
MH  - Diagnosis, Differential
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanocytes/metabolism/*pathology
MH  - Melanoma/*classification/genetics/metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Nevus/*classification/genetics/metabolism
MH  - Sensitivity and Specificity
MH  - Skin Neoplasms/*classification/genetics/metabolism
MH  - Young Adult
EDAT- 2010/01/19 06:00
MHDA- 2010/05/21 06:00
CRDT- 2010/01/19 06:00
PHST- 2010/01/19 06:00 [entrez]
PHST- 2010/01/19 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
AID - S0893-3952(22)02751-X [pii]
AID - 10.1038/modpathol.2009.177 [doi]
PST - ppublish
SO  - Mod Pathol. 2010 Mar;23(3):413-9. doi: 10.1038/modpathol.2009.177. Epub 2010 Jan 
      15.