PMID- 20084440
OWN - NLM
STAT- MEDLINE
DCOM- 20100903
LR  - 20211020
IS  - 1573-2592 (Electronic)
IS  - 0271-9142 (Linking)
VI  - 30
IP  - 2
DP  - 2010 Mar
TI  - CpG-induced Th1-type response in the downmodulation of early development of 
      allergy and inhibition of B7 expression on T cells of newborn mice.
PG  - 280-91
LID - 10.1007/s10875-009-9358-9 [doi]
AB  - INTRODUCTION: Several differences have been described between neonatal and adult 
      immune responses. The predisposition in early life to Th2-type response or 
      tolerance makes it a susceptible period for infections and allergic 
      sensitization. OBJECTIVE: The aim of this work was to evaluate the effects of 
      CpG-containing oligodeoxynucleotides on neonatal and adult immunization with 
      ovalbumin and Blomia tropicalis extract and compare the CpG effects on B and T 
      cells of neonatal and adult mice. RESULTS AND DISCUSSION: Mice that received CpG 
      showed reduced immunoglobulin E (IgE) antibody production in both neonatal and 
      adult periods, in parallel to increased IgG2a antibody levels. We observed that 
      spleen cells of mice that received CpG in early life produced increased amounts 
      of interferon-gamma upon anti-CD3 stimulation. Negative regulation of IgE 
      response was more pronounced in adult than neonate mice; further, CpG decreased 
      anaphylactic antiovalbumin IgG1 only in adults. Also, an upregulation of 
      toll-like receptor 9 expression was detected in adult B cells, but not in 
      neonatal, upon CpG stimuli. Neonatal B cells showed enhanced interleukin (IL)-10 
      expression and decreased IL-6 levels than adult B cells in response to CpG. When 
      we analyzed in vitro activation of CD4+ T cells, an increased expression of B7 
      molecules on T cells in neonates was suppressed by CpG. CONCLUSION: Altogether, 
      we verified qualitative and quantitative evidences regarding CpG effect on 
      neonatal and adult allergens immunizations, which points to the importance of 
      understanding neonatal immune system to establish immunomodulatory strategies for 
      prevention of allergic diseases.
FAU - de Brito, Cyro A
AU  - de Brito CA
AD  - Laboratory of Investigation in Dermatology and Immunodeficiencies, School of 
      Medicine, University of Sao Paulo, Sao Paulo, Brazil.
FAU - Fusaro, Ana E
AU  - Fusaro AE
FAU - Victor, Jefferson R
AU  - Victor JR
FAU - Rigato, Paula O
AU  - Rigato PO
FAU - Goldoni, Adriana L
AU  - Goldoni AL
FAU - Muniz, Bruno P
AU  - Muniz BP
FAU - Duarte, Alberto J S
AU  - Duarte AJ
FAU - Sato, Maria N
AU  - Sato MN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100119
PL  - Netherlands
TA  - J Clin Immunol
JT  - Journal of clinical immunology
JID - 8102137
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (B7-1 Antigen)
RN  - 0 (Cell Extracts)
RN  - 0 (CpG ODN 1826)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Toll-Like Receptor 9)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Antigens, Dermatophagoides/administration & dosage
MH  - B-Lymphocytes/*metabolism/pathology
MH  - B7-1 Antigen/*biosynthesis/genetics
MH  - Cell Extracts/administration & dosage
MH  - DNA/administration & dosage
MH  - Female
MH  - Hypersensitivity/blood/*immunology
MH  - Immunity, Humoral
MH  - Immunization
MH  - Immunoglobulin E/blood
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Oligodeoxyribonucleotides
MH  - Ovalbumin/administration & dosage
MH  - Pyroglyphidae
MH  - T-Lymphocytes/*metabolism/pathology
MH  - Toll-Like Receptor 9/*biosynthesis/genetics
EDAT- 2010/01/20 06:00
MHDA- 2010/09/04 06:00
CRDT- 2010/01/20 06:00
PHST- 2009/10/23 00:00 [received]
PHST- 2009/12/06 00:00 [accepted]
PHST- 2010/01/20 06:00 [entrez]
PHST- 2010/01/20 06:00 [pubmed]
PHST- 2010/09/04 06:00 [medline]
AID - 10.1007/s10875-009-9358-9 [doi]
PST - ppublish
SO  - J Clin Immunol. 2010 Mar;30(2):280-91. doi: 10.1007/s10875-009-9358-9. Epub 2010 
      Jan 19.