PMID- 20085440
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20141120
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 130
IP  - 7
DP  - 2010 Jul
TI  - In vitro cholesteatoma growth and secretion of cytokines.
PG  - 815-9
LID - 10.3109/00016480903413657 [doi]
AB  - CONCLUSION: Our results show a significant difference between skin and 
      cholesteatoma biology in vitro. OBJECTIVES: Cholesteatoma disease is a process of 
      destruction characterized by uncontrolled growth of squamous epithelial cells in 
      the middle ear or temporal bone. The pathophysiology behind the cholesteatoma 
      development is controversial, and the mechanisms driving the cholesteatoma 
      growth, migration and destructive properties is still unclear. We aimed to 
      provide a method to study the effect of various compounds on cholesteatoma and 
      skin tissue growth, as well as to further investigate the biological differences 
      between normal skin and cholesteatoma tissue. METHODS: We have established a 
      method to study cholesteatoma biopsy tissue in vitro. Cholesteatoma tissues from 
      patients undergoing surgery for chronic otitis were grown in culture medium and 
      compared to growth patterns and behaviour of normal retroauricular skin. 
      Conditioned medium was analysed for various secreted cytokines. RESULTS: We found 
      a radial outgrowth of keratinocyte epithelium from the circular biopsies. After 5 
      days of culture we found a significant growth of both cholesteatoma and 
      skin-derived cells. Cholesteatoma samples showed higher growth rate as compared 
      with skin control cultures from the same patient. Moreover, the cholesteatoma 
      cells showed higher production of monocyte chemoattractant protein-1 (MCP-1) and 
      interleukin (IL)-6 as compared with normal skin.
FAU - Helgaland, Tore
AU  - Helgaland T
AD  - Department of Otorhinolaryngology and Head and Neck Surgery, Haugesund Hospital, 
      Norway. tore.helgaland@helse-fonna.no <tore.helgaland@helse-fonna.no>
FAU - Engelen, Bart
AU  - Engelen B
FAU - Olsnes, Carla
AU  - Olsnes C
FAU - Aarstad, Hans Jorgen
AU  - Aarstad HJ
FAU - Vassbotn, Flemming S
AU  - Vassbotn FS
LA  - eng
PT  - Journal Article
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Biopsy
MH  - Chemokine CCL2/metabolism
MH  - Cholesteatoma, Middle Ear/*metabolism/*pathology
MH  - Culture Techniques
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fibroblasts/cytology
MH  - Humans
MH  - Interleukin-6/metabolism
MH  - Keratinocytes/cytology
MH  - Skin/pathology
EDAT- 2010/01/21 06:00
MHDA- 2011/03/30 06:00
CRDT- 2010/01/21 06:00
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
AID - 10.3109/00016480903413657 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2010 Jul;130(7):815-9. doi: 10.3109/00016480903413657.