PMID- 20085518
OWN - NLM
STAT- MEDLINE
DCOM- 20100316
LR  - 20100120
IS  - 1465-3931 (Electronic)
IS  - 0031-3025 (Linking)
VI  - 42
IP  - 2
DP  - 2010 Feb
TI  - PCR detectable Y chromosome-specific DNA but no intact Y chromosome-bearing cells 
      in polymyositis biopsies of two women with male offspring.
PG  - 160-4
LID - 10.3109/00313020903494953 [doi]
AB  - AIMS: Pregnancy-related and idiopathic adult polymyositis are inflammatory 
      myopathies of unknown aetiology in which CD8 positive T cells are found in close 
      association with the up-regulation of human leukocyte antigen (HLA) class I on 
      affected muscle cells. A similar polymyositis can also occur in patients with 
      chronic graft versus host disease, wherein graft lymphocytes may be involved in 
      the myositis. We investigated whether polymyositis that was temporally related to 
      pregnancy, contained Y chromosome-bearing cells or signals using polymerase chain 
      reaction (PCR) in biopsies of lesional muscle from two women who had given birth 
      to sons. Furthermore, if Y chromosome material was present, we investigated 
      whether it was contained in the intact inflammatory cells (CD8 positive 
      lymphocytes for example), fetal macrophages, or differentiated fetal stem cells 
      engrafted in the lesional skeletal muscle, and thus whether fetal cells played a 
      role in the pathogenesis of the myositis. METHODS: PCR analysis was used for the 
      Y chromosome in lesional tissue and fluorescence in situ hybridisation (FISH) for 
      intact cells carrying the Y chromosome. RESULTS: Small amounts of Y chromosome 
      material were detected on second round PCR in fresh frozen tissue. No Y 
      chromosome-bearing intact cells of lymphocytic, macrophage or muscle lineage were 
      detected. CONCLUSION: Our results suggest that microchimeric fetal cells are not 
      found in the lesional tissue of pregnancy-related polymyositis.
FAU - Fitches, Alison C
AU  - Fitches AC
AD  - Department of Pathology, University of Otago, Dunedin School of Medicine, 
      Dunedin, New Zealand.
FAU - Yousem, Samuel
AU  - Yousem S
FAU - Cieply, Kathleen
AU  - Cieply K
FAU - Stebbings, Simon
AU  - Stebbings S
FAU - Highton, John
AU  - Highton J
FAU - Hung, Noelyn A
AU  - Hung NA
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Pathology
JT  - Pathology
JID - 0175411
RN  - 0 (Sex-Determining Region Y Protein)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - CD8-Positive T-Lymphocytes/metabolism/pathology
MH  - Chimerism
MH  - Chromosomes, Human, Y/*genetics
MH  - DNA/analysis/genetics
MH  - Female
MH  - Fetus/metabolism/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Maternal-Fetal Exchange
MH  - Muscle, Skeletal/chemistry/pathology
MH  - Polymerase Chain Reaction/methods
MH  - Polymyositis/etiology/*genetics/pathology
MH  - Pregnancy
MH  - Pregnancy Complications/*genetics/pathology
MH  - Sex-Determining Region Y Protein/*genetics/metabolism
MH  - Young Adult
EDAT- 2010/01/21 06:00
MHDA- 2010/03/17 06:00
CRDT- 2010/01/21 06:00
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
AID - 10.3109/00313020903494953 [doi]
PST - ppublish
SO  - Pathology. 2010 Feb;42(2):160-4. doi: 10.3109/00313020903494953.