PMID- 20085630
OWN - NLM
STAT- MEDLINE
DCOM- 20100520
LR  - 20211020
IS  - 1465-993X (Electronic)
IS  - 1465-9921 (Print)
IS  - 1465-9921 (Linking)
VI  - 11
IP  - 1
DP  - 2010 Jan 19
TI  - Increase in markers of airway inflammation after ozone exposure can be observed 
      also in stable treated asthmatics with minimal functional response to ozone.
PG  - 5
LID - 10.1186/1465-9921-11-5 [doi]
AB  - BACKGROUND: The discrepancy between functional and inflammatory airway response 
      to ozone has been reported in normal subjects, but few data are available for 
      stable asthmatics regularly treated with inhaled corticosteroids. METHODS: 
      Twenty-three well controlled, regularly treated, mild-to-moderate asthmatic 
      patients underwent two sequential randomised exposures to either filtered air or 
      ozone (0.3 ppm for 2 hours) in a challenge chamber. Pulmonary function (PF) was 
      monitored, and patients with FEV1 decrease greater than 10% from pre-challenge 
      value were considered as responders. Immediately after each exposure, exhaled 
      breath condensate (EBC) was collected to measure malondialdehyde (MDA). Six hours 
      after each exposure, PF and EBC collection were repeated, and sputum was induced 
      to measure inflammatory cell counts and soluble mediators (IL-8 and neutrophil 
      elastase). The response to ozone was also evaluated according to the presence of 
      polymorphism in oxidative stress related NQO1 and GSTM1 genes. RESULTS: After 
      ozone exposure, sputum neutrophils significantly increased in responders (n = 8), 
      but not in nonresponders (n = 15). Other markers of neutrophil activation in 
      sputum supernatant and MDA in EBC significantly increased in all patients, but 
      only in nonresponders the increase was significant. In nonresponders, sputum 
      eosinophils also significantly increased after ozone. There was a positive 
      correlation between ozone-induced FEV1 fall and increase in sputum neutrophils. 
      No difference in functional or inflammatory response to ozone was observed 
      between subjects with or without the combination of NQO1wt- GSTM1null genotypes. 
      CONCLUSIONS: Markers of neutrophilic inflammation and oxidative stress increase 
      also in asthmatic subjects not responding to ozone. A greater functional response 
      to ozone is associated with greater neutrophil airway recruitment in asthmatic 
      subjects.
FAU - Vagaggini, Barbara
AU  - Vagaggini B
AD  - Cardio-Thoracic and Vascular Department, University of Pisa, Pisa, Italy.
FAU - Bartoli, Maria Laura E
AU  - Bartoli ML
FAU - Cianchetti, Silvana
AU  - Cianchetti S
FAU - Costa, Francesco
AU  - Costa F
FAU - Bacci, Elena
AU  - Bacci E
FAU - Dente, Federico L
AU  - Dente FL
FAU - Di Franco, Antonella
AU  - Di Franco A
FAU - Malagrino, Laura
AU  - Malagrino L
FAU - Paggiaro, Pierluigi
AU  - Paggiaro P
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20100119
PL  - England
TA  - Respir Res
JT  - Respiratory research
JID - 101090633
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 66H7ZZK23N (Ozone)
SB  - IM
MH  - Adult
MH  - Asthma/*metabolism/therapy
MH  - Biomarkers/metabolism
MH  - Cytokines/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Humans
MH  - Lung/*drug effects/*physiopathology
MH  - Male
MH  - Neutrophil Activation/*drug effects
MH  - Oxidative Stress/*drug effects
MH  - Ozone/*toxicity
PMC - PMC2817658
EDAT- 2010/01/21 06:00
MHDA- 2010/05/21 06:00
PMCR- 2010/01/19
CRDT- 2010/01/21 06:00
PHST- 2008/12/30 00:00 [received]
PHST- 2010/01/19 00:00 [accepted]
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/05/21 06:00 [medline]
PHST- 2010/01/19 00:00 [pmc-release]
AID - 1465-9921-11-5 [pii]
AID - 10.1186/1465-9921-11-5 [doi]
PST - epublish
SO  - Respir Res. 2010 Jan 19;11(1):5. doi: 10.1186/1465-9921-11-5.