PMID- 20086100
OWN - NLM
STAT- MEDLINE
DCOM- 20100409
LR  - 20211020
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 30
IP  - 6
DP  - 2010 Mar
TI  - Control of cell survival and proliferation by mammalian eukaryotic initiation 
      factor 4B.
PG  - 1478-85
LID - 10.1128/MCB.01218-09 [doi]
AB  - Translation initiation plays an important role in cell growth, proliferation, and 
      survival. The translation initiation factor eIF4B (eukaryotic initiation factor 
      4B) stimulates the RNA helicase activity of eIF4A in unwinding secondary 
      structures in the 5' untranslated region (5'UTR) of the mRNA in vitro. Here, we 
      studied the effects of eIF4B depletion in cells using RNA interference (RNAi). In 
      agreement with the role of eIF4B in translation initiation, its depletion 
      resulted in inhibition of this step. Selective reduction of translation was 
      observed for mRNAs harboring strong to moderate secondary structures in their 
      5'UTRs. These mRNAs encode proteins, which function in cell proliferation 
      (Cdc25C, c-myc, and ODC [ornithine decarboxylase]) and survival (Bcl-2 and XIAP 
      [X-linked inhibitor of apoptosis]). Furthermore, eIF4B silencing led to decreased 
      proliferation rates, promoted caspase-dependent apoptosis, and further sensitized 
      cells to camptothecin-induced cell death. These results demonstrate that eIF4B is 
      required for cell proliferation and survival by regulating the translation of 
      proliferative and prosurvival mRNAs.
FAU - Shahbazian, David
AU  - Shahbazian D
AD  - Department of Biochemistry and Goodman Cancer Centre, McGill University, The 
      Cancer Research Building, 1160 Pine Avenue West, Room 615, Montreal, Quebec H3A 
      1A3, Canada.
FAU - Parsyan, Armen
AU  - Parsyan A
FAU - Petroulakis, Emmanuel
AU  - Petroulakis E
FAU - Topisirovic, Ivan
AU  - Topisirovic I
FAU - Martineau, Yvan
AU  - Martineau Y
FAU - Gibbs, Bernard F
AU  - Gibbs BF
FAU - Svitkin, Yuri
AU  - Svitkin Y
FAU - Sonenberg, Nahum
AU  - Sonenberg N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100119
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Eukaryotic Initiation Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (eIF-4B)
RN  - EC 3.4.22.- (Caspases)
RN  - XT3Z54Z28A (Camptothecin)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Apoptosis/drug effects
MH  - Camptothecin/pharmacology
MH  - Caspases/metabolism
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Enzyme Activation/drug effects
MH  - Eukaryotic Initiation Factors/*metabolism
MH  - Gene Silencing/drug effects
MH  - HeLa Cells
MH  - Humans
MH  - Protein Biosynthesis/drug effects
MH  - RNA, Messenger/genetics/metabolism
PMC - PMC2832492
EDAT- 2010/01/21 06:00
MHDA- 2010/04/10 06:00
PMCR- 2010/09/01
CRDT- 2010/01/21 06:00
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/04/10 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - MCB.01218-09 [pii]
AID - 1218-09 [pii]
AID - 10.1128/MCB.01218-09 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2010 Mar;30(6):1478-85. doi: 10.1128/MCB.01218-09. Epub 2010 Jan 
      19.