PMID- 20086297
OWN - NLM
STAT- MEDLINE
DCOM- 20100316
LR  - 20220321
IS  - 0973-7731 (Electronic)
IS  - 0022-1333 (Linking)
VI  - 88
IP  - 3
DP  - 2009 Dec
TI  - Population distribution of 45S and 5S rDNA in golden mahseer, Tor putitora: 
      population-specific FISH marker.
PG  - 315-20
AB  - Chromosomal locations of major 45S and minor 5S ribosomal DNAs (rDNAs) and 
      organization of 5S rRNA genes were analysed in five different populations of 
      golden mahseers (Tor putitora) using fluorescence in situ hybridization (FISH) 
      and Southern blot hybridization. All five populations of T. putitora (2n = 100) 
      showed a similar type of macro-karyotype composed of 12 metacentric, 22 
      submetacentric, 14 subtelocentric and 52 telocentric chromosomes. Analysis of 
      active nucleolar organizer regions (NORs) by silver staining did not show any 
      differences in number and chromosomal position in different populations. But FISH 
      data showed significant difference between the populations, four of the five 
      populations showed six 18S (three pairs) and two 5S (one pair) signals with 
      positional polymorphism, while one population showed eight 18S and four 5S 
      signals, respectively. Southern blot data confirms that 5S rDNA clusters present 
      on two different chromosome pairs in Kosi river population contain 
      non-transcribed spacers (NTS) of same length. In the present study, simultaneous 
      localization of 45S and 5S rDNA by in situ hybridization helped us to develop the 
      discrete population-specific markers in different geographically isolated 
      populations of T. putitora.
FAU - Singh, Mamta
AU  - Singh M
AD  - National Bureau of Fish Genetic Resources, Canal Ring Road, P.O. Dilkusha, 
      Lucknow 226 002, India.
FAU - Kumar, Ravindra
AU  - Kumar R
FAU - Nagpure, N S
AU  - Nagpure NS
FAU - Kushwaha, Basdeo
AU  - Kushwaha B
FAU - Mani, Indra
AU  - Mani I
FAU - Chauhan, U K
AU  - Chauhan UK
FAU - Lakra, W S
AU  - Lakra WS
LA  - eng
PT  - Journal Article
PL  - India
TA  - J Genet
JT  - Journal of genetics
JID - 2985113R
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
RN  - 0 (RNA, Ribosomal, 5S)
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cyprinidae/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Genetic Markers
MH  - Geography
MH  - *In Situ Hybridization, Fluorescence
MH  - India
MH  - Metaphase/genetics
MH  - Population Dynamics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Species Specificity
EDAT- 2010/01/21 06:00
MHDA- 2010/03/17 06:00
CRDT- 2010/01/21 06:00
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
AID - 10.1007/s12041-009-0045-7 [doi]
PST - ppublish
SO  - J Genet. 2009 Dec;88(3):315-20. doi: 10.1007/s12041-009-0045-7.