PMID- 20087158
OWN - NLM
STAT- MEDLINE
DCOM- 20100218
LR  - 20211020
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Print)
IS  - 0147-5185 (Linking)
VI  - 34
IP  - 2
DP  - 2010 Feb
TI  - Use of fluorescence in situ hybridization (FISH) to distinguish intranodal nevus 
      from metastatic melanoma.
PG  - 231-7
LID - 10.1097/PAS.0b013e3181c805c4 [doi]
AB  - With the increase in sentinel lymph node biopsies in melanoma patients, 
      pathologists are frequently confronted with small deposits of morphologically 
      bland melanocytes in the node, which occasionally cannot be readily classified as 
      benign nodal nevi or melanoma. As most melanomas harbor characteristic 
      chromosomal aberrations which can be used to distinguish them from benign nevi, 
      we used fluorescence in-situ hybridization (FISH) with markers for 3 regions on 
      chromosome 6 and 1 on chromosome 11 to determine the presence of chromosomal 
      aberrations in sentinel lymph node specimens with small foci of melanocytes that 
      had been diagnosed as metastatic melanoma or nodal nevi by histopathology. 
      Fifty-nine tissue samples from 41 patients (24 lymph node metastases, 17 with 
      nodal nevi, and 18 of the available corresponding primary melanomas) were 
      analyzed by FISH. Twenty of 24 (83%) cases diagnosed as metastatic melanoma 
      showed aberrations by FISH. Of the 4 negative cases, 3 were unequivocal melanoma 
      metastases, whereas 1 on re-review was histopathologically equivocal. Of the 17 
      nodal nevi, 1 (6%) also showed aberrations by FISH, whereas the remainder was 
      negative. Multiple aberrations were present in the positive case, some of which 
      were also found in the corresponding primary tumor, suggesting that this case 
      represents a deceptively bland melanoma metastasis that had been misclassified by 
      histomorphology. Our data indicate that FISH is a useful adjunct tool to 
      traditional methods in the diagnostic workup of deposits of melanocytes in lymph 
      nodes that are histopathologically ambiguous.
FAU - Dalton, Scott R
AU  - Dalton SR
AD  - Department of Pathology, Wilford Hall Medical Center, Lackland AFB, TX, USA.
FAU - Gerami, Pedram
AU  - Gerami P
FAU - Kolaitis, Nicholas A
AU  - Kolaitis NA
FAU - Charzan, Susan
AU  - Charzan S
FAU - Werling, Rob
AU  - Werling R
FAU - LeBoit, Philip E
AU  - LeBoit PE
FAU - Bastian, Boris C
AU  - Bastian BC
LA  - eng
GR  - R01 CA131524/CA/NCI NIH HHS/United States
GR  - R01 CA131524-01A1/CA/NCI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
SB  - IM
MH  - Chromosome Aberrations
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymph Nodes/*pathology
MH  - Lymphatic Metastasis/diagnosis
MH  - Melanocytes/pathology
MH  - Melanoma/*diagnosis/genetics
MH  - Nevus, Pigmented/*diagnosis
MH  - Skin Neoplasms/*diagnosis/genetics
PMC - PMC2831773
MID - NIHMS175443
EDAT- 2010/01/21 06:00
MHDA- 2010/02/19 06:00
PMCR- 2011/02/01
CRDT- 2010/01/21 06:00
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/02/19 06:00 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - 10.1097/PAS.0b013e3181c805c4 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2010 Feb;34(2):231-7. doi: 10.1097/PAS.0b013e3181c805c4.