PMID- 20087752
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20211020
IS  - 1420-9071 (Electronic)
IS  - 1420-682X (Linking)
VI  - 67
IP  - 8
DP  - 2010 Apr
TI  - Selective effect of burn injury on splenic CD11c(+) dendritic cells and 
      CD8alpha(+)CD4(-)CD11c(+) dendritic cell subsets.
PG  - 1315-29
LID - 10.1007/s00018-009-0251-8 [doi]
AB  - Burn injury causes an immunosuppression associated with suppressed adaptive 
      immune function. Dendritic cells (DCs) are APCs for which signaling via their 
      Toll-like receptors (TLRs) induces their maturation and activation, which is 
      essential for the adaptive immune response. In this study, we examined if burn 
      injury alters the TLR activity of splenic DCs. After injury, we noticed that DC 
      functions were impaired, characterized by a suppressed capacity to prime naive T 
      cells when triggering the TLR4 signaling cascade using specific ligands (LPS or 
      rHSP60). The observed perturbations on LPS-primed DCs isolated from burned mice 
      exhibited significantly diminished IL-12p40 production and enhanced IL-10 
      secretion-associated impairment in mitogen-activated protein kinase activation. 
      Interestingly, we observed a decrease of TLR4/MD-2 expression on the CD8alpha(+) 
      DC subset that persisted following LPS stimulation. The altered TLR4 expression 
      on LPS-stimulated CD8alpha(+) DCs was associated with reduced capacity to produce 
      IL-12 after stimulation. Our results suggested that TLR4 reactivity on DCs, 
      especially CD8alpha(+) DCs, is disturbed after burn injury.
FAU - Patenaude, Julie
AU  - Patenaude J
AD  - INRS-Institut Armand-Frappier, Laval, QC, Canada.
FAU - D'Elia, Michele
AU  - D'Elia M
FAU - Hamelin, Claudine
AU  - Hamelin C
FAU - Bernier, Jacques
AU  - Bernier J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100120
PL  - Switzerland
TA  - Cell Mol Life Sci
JT  - Cellular and molecular life sciences : CMLS
JID - 9705402
RN  - 0 (CD11c Antigen)
RN  - 0 (CD4 Antigens)
RN  - 0 (CD8 Antigens)
RN  - 0 (CD8alpha antigen)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (Toll-Like Receptor 4)
RN  - 130068-27-8 (Interleukin-10)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Burns/*metabolism
MH  - CD11c Antigen/*metabolism
MH  - CD4 Antigens/*metabolism
MH  - CD8 Antigens/metabolism
MH  - Dendritic Cells/*physiology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Interleukin-10/metabolism
MH  - Interleukin-12 Subunit p40/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mitogen-Activated Protein Kinases/metabolism
MH  - Phosphorylation
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spleen/cytology
MH  - T-Lymphocytes/drug effects/metabolism
MH  - Toll-Like Receptor 4/metabolism
EDAT- 2010/01/21 06:00
MHDA- 2010/04/14 06:00
CRDT- 2010/01/21 06:00
PHST- 2009/08/11 00:00 [received]
PHST- 2009/12/23 00:00 [accepted]
PHST- 2009/12/10 00:00 [revised]
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - 10.1007/s00018-009-0251-8 [doi]
PST - ppublish
SO  - Cell Mol Life Sci. 2010 Apr;67(8):1315-29. doi: 10.1007/s00018-009-0251-8. Epub 
      2010 Jan 20.