PMID- 20087882
OWN - NLM
STAT- MEDLINE
DCOM- 20100422
LR  - 20220408
IS  - 1872-8081 (Electronic)
IS  - 0951-6433 (Linking)
VI  - 36
IP  - 1
DP  - 2010 Jan-Feb
TI  - A fraction of unripe kiwi fruit extract regulates adipocyte differentiation and 
      function in 3T3-L1 cells.
PG  - 52-9
LID - 10.1002/biof.70 [doi]
AB  - Adipocyte dysfunction is strongly associated with the development of insulin 
      resistance and diabetes, and regulation of adipogenesis is important in 
      prevention of diabetes. We prepared a 100% methanol fraction of methanolic 
      extract from unripe kiwi fruit (Actinidia deliciosa), designated KMF (kiwi fruit 
      methanol fraction). When applied to 3T3-L1 preadipocyte cells, KMF promoted 
      adipocyte differentiation, increased glycerol-3-phosphate dehydrogenase (GPDH) 
      activity, and increased triglyceride (TG) content. KMF markedly increased mRNA 
      expression of peroxisome proliferator-activated receptor gamma (PPARgamma)-the 
      master adipogenic transcription factor-and its target genes. Moreover, KMF 
      increased mRNA expression and protein secretion of adiponectin, whereas mRNA 
      expression and secretion of monocyte chemoattractant protein-1 (MCP-1) and 
      interleukin-6 (IL-6) were decreased. Compared with troglitazone, KMF decreased 
      the production of reactive oxygen species (ROS) and nuclear factor-kappaB 
      (NFkappaB) activation. Glucose uptake was stimulated by KMF in differentiated 
      3T3-L1 adipocytes. Taken together, these results indicate that KMF may exert 
      beneficial effects against diabetes via its ability to regulate adipocyte 
      differentiation and function.
FAU - Abe, Daigo
AU  - Abe D
AD  - National Agricultural Research Center for Western Region, Zentsuji, Kagawa, 
      Japan.
FAU - Saito, Takeshi
AU  - Saito T
FAU - Kubo, Yasutaka
AU  - Kubo Y
FAU - Nakamura, Yoshimasa
AU  - Nakamura Y
FAU - Sekiya, Keizo
AU  - Sekiya K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biofactors
JT  - BioFactors (Oxford, England)
JID - 8807441
RN  - 0 (Adiponectin)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chromans)
RN  - 0 (Interleukin-6)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Thiazolidinediones)
RN  - 0 (Triglycerides)
RN  - EC 1.1.- (Glycerolphosphate Dehydrogenase)
RN  - I66ZZ0ZN0E (Troglitazone)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Actinidia/*chemistry
MH  - Adipocytes/cytology/drug effects/*physiology
MH  - Adipogenesis/drug effects
MH  - Adiponectin/biosynthesis
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Chemokine CCL2/metabolism
MH  - Chromans/pharmacology
MH  - Glucose/metabolism
MH  - Glycerolphosphate Dehydrogenase/metabolism
MH  - Interleukin-6/metabolism
MH  - Mice
MH  - NF-kappa B/physiology
MH  - Plant Extracts/*pharmacology
MH  - RNA, Messenger/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Thiazolidinediones/pharmacology
MH  - Triglycerides/metabolism
MH  - Troglitazone
EDAT- 2010/01/21 06:00
MHDA- 2010/04/23 06:00
CRDT- 2010/01/21 06:00
PHST- 2010/01/21 06:00 [entrez]
PHST- 2010/01/21 06:00 [pubmed]
PHST- 2010/04/23 06:00 [medline]
AID - 10.1002/biof.70 [doi]
PST - ppublish
SO  - Biofactors. 2010 Jan-Feb;36(1):52-9. doi: 10.1002/biof.70.