PMID- 20089406
OWN - NLM
STAT- MEDLINE
DCOM- 20100812
LR  - 20161125
IS  - 1532-2653 (Electronic)
IS  - 0967-5868 (Linking)
VI  - 17
IP  - 6
DP  - 2010 Jun
TI  - Expression of soluble Nogo-66 receptor and brain-derived neurotrophic factor in 
      transduced rat bone marrow stromal cells.
PG  - 762-5
LID - 10.1016/j.jocn.2009.08.017 [doi]
AB  - Bone marrow stromal cells (BMSCs) are promising candidates for cell 
      transplantation in the central nervous system. When grafted into injury sites, 
      they may be able to form 'guiding strands' for host axonal growth, and secrete 
      nerve growth factor and brain-derived neurotrophic factor (BDNF) to support 
      injured neurons and axons. However, they have no effect on the inhibitory 
      molecules secreted locally following neuronal injury. The Nogo-66 receptor (NgR) 
      plays a key role in inhibiting axon regeneration in the central nervous system. 
      Exogenous soluble NgR can competitively bind to inhibitors and improve locomotor 
      function recovery. In this study, a gene encoding soluble NgR was cloned and 
      transduced into rat BMSCs using a lentiviral vector. Expression of soluble NgR 
      was detected in the rat BMSCs. NgR-expressing BMSCs also secreted BDNF during 
      culture in vitro. These results indicate that transduced BMSCs not only 
      antagonize the effects of molecules inhibiting axon growth but also express 
      neurotrophic factors, and thus have the potential to promote axon regeneration 
      via more than one mechanism.
CI  - Copyright 2009 Elsevier Ltd. All rights reserved.
FAU - Liu, Min
AU  - Liu M
AD  - Department of Microbiology, Wuhan University School of Medicine, Wuhan, China.
FAU - Ma, Yonggang
AU  - Ma Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100120
PL  - Scotland
TA  - J Clin Neurosci
JT  - Journal of clinical neuroscience : official journal of the Neurosurgical Society 
      of Australasia
JID - 9433352
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Myelin Proteins)
RN  - 0 (Nogo Proteins)
RN  - 0 (Nogo Receptor 1)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Rtn4 protein, rat)
RN  - 0 (Rtn4r protein, rat)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*metabolism
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - GPI-Linked Proteins
MH  - Male
MH  - Myelin Proteins/genetics/*metabolism
MH  - Nogo Proteins
MH  - Nogo Receptor 1
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/genetics/metabolism
MH  - Stromal Cells/*metabolism
MH  - Transduction, Genetic/methods
EDAT- 2010/01/22 06:00
MHDA- 2010/08/13 06:00
CRDT- 2010/01/22 06:00
PHST- 2009/07/07 00:00 [received]
PHST- 2009/08/28 00:00 [revised]
PHST- 2009/08/30 00:00 [accepted]
PHST- 2010/01/22 06:00 [entrez]
PHST- 2010/01/22 06:00 [pubmed]
PHST- 2010/08/13 06:00 [medline]
AID - S0967-5868(09)00686-9 [pii]
AID - 10.1016/j.jocn.2009.08.017 [doi]
PST - ppublish
SO  - J Clin Neurosci. 2010 Jun;17(6):762-5. doi: 10.1016/j.jocn.2009.08.017. Epub 2010 
      Jan 20.