PMID- 20089706
OWN - NLM
STAT- MEDLINE
DCOM- 20100326
LR  - 20210103
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 184
IP  - 4
DP  - 2010 Feb 15
TI  - Peroxynitrite-dependent killing of cancer cells and presentation of released 
      tumor antigens by activated dendritic cells.
PG  - 1876-84
LID - 10.4049/jimmunol.0900831 [doi]
AB  - Dendritic cells (DCs), essential for the initiation and regulation of adaptive 
      immune responses, have been used as anticancer vaccines. DCs may also directly 
      trigger tumor cell death. In the current study, we have investigated the 
      tumoricidal and immunostimulatory activities of mouse bone marrow-derived DCs. 
      Our results indicate that these cells acquire killing capabilities toward tumor 
      cells only when activated with LPS or Pam3Cys-SK4. Using different transgenic 
      mouse models including inducible NO synthase or GP91 knockout mice, we have 
      further established that LPS- or Pam3Cys-SK4-activated DC killing activity 
      involves peroxynitrites. Importantly, after killing of cancer cells, DCs are 
      capable of engulfing dead tumor cell fragments and of presenting tumor Ags to 
      specific T lymphocytes. Thus, upon specific stimulation, mouse bone 
      marrow-derived DCs can directly kill tumor cells through a novel 
      peroxynitrite-dependent mechanism and participate at virtually all levels of 
      antitumor immune responses, which reinforces their interest in immunotherapy.
FAU - Fraszczak, Jennifer
AU  - Fraszczak J
AD  - Institut National de la Sante et de la Recherche Medicale Unite Mixte de 
      Recherche 866, Institut de Recherche Federatif 100, Universite de Bourgogne, 
      Dijon, France.
FAU - Trad, Malika
AU  - Trad M
FAU - Janikashvili, Nona
AU  - Janikashvili N
FAU - Cathelin, Dominique
AU  - Cathelin D
FAU - Lakomy, Daniela
AU  - Lakomy D
FAU - Granci, Virginie
AU  - Granci V
FAU - Morizot, Alexandre
AU  - Morizot A
FAU - Audia, Sylvain
AU  - Audia S
FAU - Micheau, Olivier
AU  - Micheau O
FAU - Lagrost, Laurent
AU  - Lagrost L
FAU - Katsanis, Emmanuel
AU  - Katsanis E
FAU - Solary, Eric
AU  - Solary E
FAU - Larmonier, Nicolas
AU  - Larmonier N
FAU - Bonnotte, Bernard
AU  - Bonnotte B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100120
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, Neoplasm)
RN  - 14691-52-2 (Peroxynitrous Acid)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Antigens, Neoplasm/*immunology/*metabolism
MH  - Cell Death/immunology
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Dendritic Cells/*immunology/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Melanoma, Experimental
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Neoplasms, Experimental/*immunology/metabolism/*pathology
MH  - Peroxynitrous Acid/*toxicity
EDAT- 2010/01/22 06:00
MHDA- 2010/03/27 06:00
CRDT- 2010/01/22 06:00
PHST- 2010/01/22 06:00 [entrez]
PHST- 2010/01/22 06:00 [pubmed]
PHST- 2010/03/27 06:00 [medline]
AID - jimmunol.0900831 [pii]
AID - 10.4049/jimmunol.0900831 [doi]
PST - ppublish
SO  - J Immunol. 2010 Feb 15;184(4):1876-84. doi: 10.4049/jimmunol.0900831. Epub 2010 
      Jan 20.