PMID- 20089896
OWN - NLM
STAT- MEDLINE
DCOM- 20100205
LR  - 20211020
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Print)
IS  - 0270-6474 (Linking)
VI  - 30
IP  - 3
DP  - 2010 Jan 20
TI  - TRPV1 gene deficiency attenuates miniature EPSC potentiation induced by mannitol 
      and angiotensin II in supraoptic magnocellular neurons.
PG  - 876-84
LID - 10.1523/JNEUROSCI.2986-09.2010 [doi]
AB  - The release of arginine vasopressin (AVP) from the magnocellular neurosecretory 
      cells (MNCs) in the supraoptic nucleus (SON) is crucial for body fluid 
      homeostasis. The MNC activity is modulated by synaptic inputs and humoral 
      factors. A recent study demonstrated that an N-terminal splice variant of the 
      transient receptor potential vanilloid type 1 (TRPV1) is essential for 
      osmosensory transduction in the SON. In the present study, we examined the 
      effects of mannitol and angiotensin II on miniature EPSCs (mEPSCs) in the 
      supraoptic MNCs using whole-cell patch-clamp recording in in vitro slice 
      preparation. Mannitol (60 mm) and angiotensin II (0.1 microm) increased the 
      frequency of mEPSCs without affecting the amplitude. These effects were 
      attenuated by pre-exposure to a nonspecific TRPV channel blocker, ruthenium red 
      (10 microm) and enhanced by pre-exposure to cannabinoid type1 receptor 
      antagonist, AM251 (2 microm). Mannitol-induced potentiation of mEPSCs was not 
      attenuated by angiotensin II receptor antagonist, losartan (10 microm), 
      indicating independent pathways of mannitol and angiotensin II to the TRPV 
      channels. The potentiation of mEPSCs by mannitol was not mimicked by a TRPV1 
      agonist, capsaicin, and also not attenuated by TRPV1 blockers, capsazepine (10 
      microm). PKC was involved in angiotensin II-induced potentiation of mEPSCs. The 
      effects of mannitol and angiotensin II on the supraoptic MNCs in trpv1 knock-out 
      mice were significantly attenuated compared with those in wild-type mice 
      counterparts. The results suggest that hyperosmotic stimulation and angiotensin 
      II independently modulate mEPSCs through capsaicin-insensitive TRPV1 channel in 
      the presynaptic terminals of the SON.
FAU - Yokoyama, Toru
AU  - Yokoyama T
AD  - Department of Physiology, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu 807-8555, Japan.
FAU - Saito, Takeshi
AU  - Saito T
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
FAU - Hashimoto, Hirofumi
AU  - Hashimoto H
FAU - Suzuki, Hitoshi
AU  - Suzuki H
FAU - Otsubo, Hiroki
AU  - Otsubo H
FAU - Fujihara, Hiroaki
AU  - Fujihara H
FAU - Nagatomo, Toshihisa
AU  - Nagatomo T
FAU - Ueta, Yoichi
AU  - Ueta Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Anilides)
RN  - 0 (Chelating Agents)
RN  - 0 (Cinnamates)
RN  - 0 (Diuretics, Osmotic)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (N-(3-methoxyphenyl)-4-chlorocinnamanilide)
RN  - 0 
      (N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carboxamide)
RN  - 0 (Piperidines)
RN  - 0 (Pyrazines)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyridines)
RN  - 0 (TRPV Cation Channels)
RN  - 0 (TRPV1 protein, mouse)
RN  - 0 (Vasoconstrictor Agents)
RN  - 11128-99-7 (Angiotensin II)
RN  - 3I4FA44MAI (AM 251)
RN  - 3OWL53L36A (Mannitol)
RN  - 526U7A2651 (Egtazic Acid)
RN  - K22DDW77C0 (1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)
RN  - LFW48MY844 (capsazepine)
RN  - S07O44R1ZM (Capsaicin)
SB  - IM
MH  - Analysis of Variance
MH  - Angiotensin II/*pharmacology
MH  - Anilides/pharmacology
MH  - Animals
MH  - Capsaicin/analogs & derivatives/pharmacology
MH  - Chelating Agents/pharmacology
MH  - Cinnamates/pharmacology
MH  - Diuretics, Osmotic/*pharmacology
MH  - Drug Interactions
MH  - Egtazic Acid/analogs & derivatives/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - In Vitro Techniques
MH  - Male
MH  - Mannitol/*pharmacology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Miniature Postsynaptic Potentials/*drug effects/*genetics
MH  - Neurons/*drug effects
MH  - Piperidines/pharmacology
MH  - Pyrazines/pharmacology
MH  - Pyrazoles/pharmacology
MH  - Pyridines/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/drug effects
MH  - Supraoptic Nucleus/*cytology
MH  - TRPV Cation Channels/*deficiency
MH  - Vasoconstrictor Agents/*pharmacology
PMC - PMC6633090
EDAT- 2010/01/22 06:00
MHDA- 2010/02/06 06:00
PMCR- 2010/07/20
CRDT- 2010/01/22 06:00
PHST- 2010/01/22 06:00 [entrez]
PHST- 2010/01/22 06:00 [pubmed]
PHST- 2010/02/06 06:00 [medline]
PHST- 2010/07/20 00:00 [pmc-release]
AID - 30/3/876 [pii]
AID - 3561700 [pii]
AID - 10.1523/JNEUROSCI.2986-09.2010 [doi]
PST - ppublish
SO  - J Neurosci. 2010 Jan 20;30(3):876-84. doi: 10.1523/JNEUROSCI.2986-09.2010.