PMID- 20090303
OWN - NLM
STAT- MEDLINE
DCOM- 20101103
LR  - 20161125
IS  - 1422-6421 (Electronic)
IS  - 1422-6405 (Linking)
VI  - 191
IP  - 6
DP  - 2010
TI  - Development of rat tibia innervation: colocalization of autonomic nerve fiber 
      markers with growth-associated protein 43.
PG  - 489-99
LID - 10.1159/000276591 [doi]
AB  - Development of autonomic innervation of the tibia was investigated in rat fetuses 
      on gestational days (GD) 17-21 and in juvenile animals on postnatal days (PD) 
      1-28. Double immunofluorescence combined with confocal microscopy was applied to 
      study colocalization of neuronal growth- associated protein 43 (GAP-43) and 
      panneuronal marker protein gene product 9.5 (PGP) with markers of the autonomic 
      nervous system: neuropeptide Y (NPY) and dopamine beta-hydroxylase (DbetaH) for 
      adrenergic, as well as vasoactive intestinal polypeptide (VIP) and vesicular 
      acetylcholine transporter (VAChT) for cholinergic fibers. The first 
      GAP-43-immunoreactive (GAP-IR) nerve fibers were seen on GD17 in the 
      perichondrium of the proximal epiphysis. Further GAP- and PGP-IR innervation 
      appeared in the perichondrium/periosteum of the diaphysis and in the distal 
      epiphysis (GD19), then in the bone marrow and in the intercondylar eminence 
      (GD21). On PD1, NPY-IR and DbetaH-IR fibers appeared within the diaphyseal 
      periosteum and on PD4 within the bone marrow. From PD14, GAP-43 immunoreactivity 
      of NPY-positive fibers decreased. From PD7 on, NPY-IR fibers were observed in 
      cartilage canals of both epiphyses and in the intercondylar eminence. In 
      secondary ossification centers, NPY-IR fibers were seen from PD10, and in the 
      bone marrow of the epiphyses from PD14. First VIP-IR and VAChT-IR fibers were 
      observed on PD4 within the periosteum, bone marrow and patellar ligament. From 
      PD10 on, VIP-positive fibers were seen in the intercondylar eminence, and from 
      PD14 in secondary ossification centers. GAP-43 proved to be superior to PGP 9.5 
      as marker of growing nerve fibers, mostly due to its earlier appearance. The 
      presence of specific nerve fibers may suggest possible involvement of autonomic 
      innervation in regulation of bone development.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Gajda, Mariusz
AU  - Gajda M
AD  - Department of Histology, Jagiellonian University Medical College, Krakow, Poland. 
      mmgajda @ cyf-kr.edu.pl
FAU - Litwin, Jan A
AU  - Litwin JA
FAU - Tabarowski, Zbigniew
AU  - Tabarowski Z
FAU - Zagolski, Olaf
AU  - Zagolski O
FAU - Cichocki, Tadeusz
AU  - Cichocki T
FAU - Timmermans, Jean-Pierre
AU  - Timmermans JP
FAU - Adriaensen, Dirk
AU  - Adriaensen D
LA  - eng
PT  - Journal Article
DEP - 20100114
PL  - Switzerland
TA  - Cells Tissues Organs
JT  - Cells, tissues, organs
JID - 100883360
RN  - 0 (GAP-43 Protein)
RN  - 0 (Neuropeptide Y)
RN  - 0 (Vesicular Acetylcholine Transport Proteins)
RN  - 37221-79-7 (Vasoactive Intestinal Peptide)
RN  - EC 3.4.19.12 (UCHL1 protein, rat)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Animals
MH  - Female
MH  - GAP-43 Protein/*metabolism
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Models, Biological
MH  - Nerve Fibers/*metabolism
MH  - Neuropeptide Y/metabolism
MH  - Pregnancy
MH  - Rats
MH  - Rats, Wistar
MH  - Tibia/*innervation
MH  - Time Factors
MH  - Ubiquitin Thiolesterase/metabolism
MH  - Vasoactive Intestinal Peptide/metabolism
MH  - Vesicular Acetylcholine Transport Proteins/metabolism
EDAT- 2010/01/22 06:00
MHDA- 2010/11/04 06:00
CRDT- 2010/01/22 06:00
PHST- 2009/10/03 00:00 [accepted]
PHST- 2010/01/22 06:00 [entrez]
PHST- 2010/01/22 06:00 [pubmed]
PHST- 2010/11/04 06:00 [medline]
AID - 000276591 [pii]
AID - 10.1159/000276591 [doi]
PST - ppublish
SO  - Cells Tissues Organs. 2010;191(6):489-99. doi: 10.1159/000276591. Epub 2010 Jan 
      14.