PMID- 20090787
OWN - NLM
STAT- MEDLINE
DCOM- 20100713
LR  - 20100420
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Linking)
VI  - 4
IP  - 5
DP  - 2010 May
TI  - Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy 
      sediments in the Straits of Florida.
PG  - 686-99
LID - 10.1038/ismej.2009.149 [doi]
AB  - Marine sediments and sponges may show steep variations in redox potential, 
      providing niches for both aerobic and anaerobic microorganisms. Geodia spp. and 
      sediment specimens from the Straits of Florida were fixed using paraformaldehyde 
      and 95% ethanol (v/v) for fluorescence in situ hybridization (FISH). In addition, 
      homogenates of sponge and sediment samples were incubated anaerobically on 
      various cysteine supplemented agars. FISH analysis showed a prominent similarity 
      of microbiota in sediments and Geodia spp. samples. Furthermore, the presence of 
      sulfate-reducing and annamox bacteria as well as other obligate anaerobic 
      microorganisms in both Geodia spp. and sediment samples were also confirmed. 
      Anaerobic cultures obtained from the homogenates allowed the isolation of a 
      variety of facultative anaerobes, primarily Bacillus spp. and Vibrio spp. 
      Obligate anaerobes such as Desulfovibrio spp. and Clostridium spp. were also 
      found. We also provide the first evidence for a culturable marine member of the 
      Chloroflexi, which may enter into symbiotic relationships with deep-water sponges 
      such as Geodia spp. Resuspended sediment particles, may provide a source of 
      microorganisms able to associate or form a symbiotic relationship with sponges.
FAU - Bruck, Wolfram M
AU  - Bruck WM
AD  - Center for Marine Biomedical and Biotechnology Research, Florida Atlantic 
      University, Fort Pierce, FL, USA.
FAU - Bruck, Thomas B
AU  - Bruck TB
FAU - Self, William T
AU  - Self WT
FAU - Reed, John K
AU  - Reed JK
FAU - Nitecki, Sonja S
AU  - Nitecki SS
FAU - McCarthy, Peter J
AU  - McCarthy PJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100121
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
SB  - IM
MH  - Animals
MH  - Bacteria, Anaerobic/*classification/*isolation & purification
MH  - Florida
MH  - Geodia/*microbiology
MH  - Geologic Sediments/*microbiology
MH  - In Situ Hybridization, Fluorescence
EDAT- 2010/01/22 06:00
MHDA- 2010/07/14 06:00
CRDT- 2010/01/22 06:00
PHST- 2010/01/22 06:00 [entrez]
PHST- 2010/01/22 06:00 [pubmed]
PHST- 2010/07/14 06:00 [medline]
AID - ismej2009149 [pii]
AID - 10.1038/ismej.2009.149 [doi]
PST - ppublish
SO  - ISME J. 2010 May;4(5):686-99. doi: 10.1038/ismej.2009.149. Epub 2010 Jan 21.