PMID- 20093228
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20140408
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 133
IP  - 2
DP  - 2010 Feb
TI  - Comparison of fluorescence in situ hybridization, p57 immunostaining, flow 
      cytometry, and digital image analysis for diagnosing molar and nonmolar products 
      of conception.
PG  - 196-204
LID - 10.1309/AJCPV7BRDUCX0WAQ [doi]
AB  - Pathologic examination of products of conception (POC) is used to differentiate 
      hydropic abortus (HA), partial hydatidiform mole (PM), and complete hydatidiform 
      mole (CM). Histologic classification of POC specimens can be difficult, and 
      ancillary testing is often required for a definitive diagnosis. This study 
      evaluated 66 POC specimens by flow cytometry, digital image analysis, p57 
      immunohistochemical analysis, and fluorescence in situ hybridization (FISH). The 
      final diagnosis, based on the combined analysis of all test results, included 33 
      HAs, 24 PMs, and 9 CMs. The p57 immunostain identified 9 CMs that were evaluated 
      as nontriploid by all other techniques. FISH seems to have the best accuracy 
      (100%) for determining whether a specimen contains a triploid chromosome 
      complement. These data suggest that the combination of p57 and FISH seems to be 
      the best ancillary testing strategy to aid pathologists in the appropriate 
      identification of CM, PM, and HA in POC specimens.
FAU - Kipp, Benjamin R
AU  - Kipp BR
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, USA.
FAU - Ketterling, Rhett P
AU  - Ketterling RP
FAU - Oberg, Trynda N
AU  - Oberg TN
FAU - Cousin, Margot A
AU  - Cousin MA
FAU - Plagge, Amy M
AU  - Plagge AM
FAU - Wiktor, Anne E
AU  - Wiktor AE
FAU - Ihrke, Johnita M
AU  - Ihrke JM
FAU - Meyers, Cecelia H
AU  - Meyers CH
FAU - Morice, William G
AU  - Morice WG
FAU - Halling, Kevin C
AU  - Halling KC
FAU - Clayton, Amy C
AU  - Clayton AC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (CDKN1C protein, human)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p57)
SB  - IM
MH  - Abortion, Induced
MH  - Adult
MH  - Cyclin-Dependent Kinase Inhibitor p57/*analysis
MH  - Diagnosis, Differential
MH  - *Diagnostic Imaging
MH  - Female
MH  - *Flow Cytometry
MH  - Humans
MH  - Hydatidiform Mole/diagnosis/*genetics
MH  - *Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - *Ploidies
MH  - Pregnancy
EDAT- 2010/01/23 06:00
MHDA- 2010/02/18 06:00
CRDT- 2010/01/23 06:00
PHST- 2010/01/23 06:00 [entrez]
PHST- 2010/01/23 06:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
AID - 133/2/196 [pii]
AID - 10.1309/AJCPV7BRDUCX0WAQ [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2010 Feb;133(2):196-204. doi: 10.1309/AJCPV7BRDUCX0WAQ.