PMID- 20093229
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20171116
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 133
IP  - 2
DP  - 2010 Feb
TI  - Visualization of FISH Probes by dual-color chromogenic in situ hybridization.
PG  - 205-11
LID - 10.1309/AJCP12MHRTFZJPKW [doi]
AB  - The overall purpose of the study was to demonstrate applicability of the DAKO 
      dual-color chromogenic in situ hybridization (CISH) assay (DAKO Denmark, 
      Glostrup) with respect to 4 fluorescence in situ hybridization (FISH) probes: MYC 
      (c-MYC), EGFR, ERBB2 (HER2), and TOP2A. The study showed that the dual-color CISH 
      assay can convert Texas red and fluorescein isothiocyanate (FITC) signals into 
      chromogenic signals with an almost complete 1:1 conversion ratio. Agreement 
      studies between the FISH assays for HER2 and TOP2A and the corresponding CISH 
      conversion assays showed 100% concordance (kappa values of 1.0) between the CISH 
      and FISH methods for HER2 and TOP2A status. The correlations of the gene copy 
      number to centromere-17 ratios were similarly high, with a correlation 
      coefficient (r) for HER2 and TOP2A of more than 0.95. Owing to the relatively 
      small number of specimens in this study, it is important that the data are 
      confirmed in a larger study.
FAU - Hoff, Kirsten
AU  - Hoff K
AD  - Antibody Development, CMC Contrast, Copenhagen, Denmark.
FAU - Jorgensen, Jan T
AU  - Jorgensen JT
FAU - Muller, Sven
AU  - Muller S
FAU - Rongaard, Else
AU  - Rongaard E
FAU - Rasmussen, Ole
AU  - Rasmussen O
FAU - Schonau, Andreas
AU  - Schonau A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Chromogenic Compounds)
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Poly-ADP-Ribose Binding Proteins)
RN  - EC 5.99.1.3 (DNA Topoisomerases, Type II)
RN  - EC 5.99.1.3 (TOP2A protein, human)
SB  - IM
CIN - Am J Clin Pathol. 2010 Aug;134(2):347-8. PMID: 20660343
MH  - Antigens, Neoplasm/genetics
MH  - *Chromogenic Compounds
MH  - *DNA Probes
MH  - DNA Topoisomerases, Type II/genetics
MH  - DNA-Binding Proteins/genetics
MH  - Gene Dosage
MH  - Genes, erbB-2
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Poly-ADP-Ribose Binding Proteins
EDAT- 2010/01/23 06:00
MHDA- 2010/02/18 06:00
CRDT- 2010/01/23 06:00
PHST- 2010/01/23 06:00 [entrez]
PHST- 2010/01/23 06:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
AID - 133/2/205 [pii]
AID - 10.1309/AJCP12MHRTFZJPKW [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2010 Feb;133(2):205-11. doi: 10.1309/AJCP12MHRTFZJPKW.