PMID- 20093390
OWN - NLM
STAT- MEDLINE
DCOM- 20100707
LR  - 20240420
IS  - 1943-7811 (Electronic)
IS  - 1525-1578 (Print)
IS  - 1525-1578 (Linking)
VI  - 12
IP  - 2
DP  - 2010 Mar
TI  - Multiplex ligation-dependent probe amplification versus multiprobe fluorescence 
      in situ hybridization to detect genomic aberrations in chronic lymphocytic 
      leukemia: a tertiary center experience.
PG  - 197-203
LID - 10.2353/jmoldx.2010.090046 [doi]
AB  - Cytogenetic abnormalities play a major role in the prognosis of patients with 
      chronic lymphocytic leukemia (CLL). Several methods have emerged to try to best 
      identify these abnormalities. We used fluorescence in situ hybridization (FISH) 
      to determine the frequency of cytogenetic changes in our CLL patient population. 
      We also evaluated the effectiveness of multiplex ligation-dependent probe 
      amplification (MLPA) in detecting these abnormalities. Sixty-two B-CLL patients 
      and 20 healthy controls were enrolled, and FISH and MLPA analyses were performed 
      on peripheral blood samples. Using FISH, genomic aberrations were found in 73% of 
      patients and presented as follows: single 13q14.3 deletion (60%), trisomy 12 
      (7%), ATM deletion (6%), 17p13.1 deletion (2%). MLPA analyses done on 61/62 
      patients showed sensitivity and specificity values of 90% and 100% respectively. 
      MLPA revealed several additional copy number changes, the most common being 19p13 
      (LDLR and CDKN2D). Moreover, the cost for MLPA analysis, including technical time 
      and reagents, is 86% less than FISH. In conclusion, cytogenetic abnormalities are 
      a common finding in CLL patients, and MLPA is a reliable approach that is more 
      cost effective and faster than FISH. Despite MLPA limitations of sensitivity, it 
      can be used as a first-line screen and complementary test to FISH analysis.
FAU - Al Zaabi, Eiman A
AU  - Al Zaabi EA
AD  - Department of Pathology, Division of Hematopathology, 5788 University Avenue, 
      Mackenzie Building, Capital District Health Authority, Halifax, NS, B3H 1V8 
      Canada.
FAU - Fernandez, Louis A
AU  - Fernandez LA
FAU - Sadek, Irene A
AU  - Sadek IA
FAU - Riddell, D Christie
AU  - Riddell DC
FAU - Greer, Wenda L
AU  - Greer WL
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100121
PL  - United States
TA  - J Mol Diagn
JT  - The Journal of molecular diagnostics : JMD
JID - 100893612
SB  - IM
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods
MH  - Karyotyping/methods
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis/genetics
MH  - Male
MH  - Middle Aged
MH  - Nucleic Acid Amplification Techniques/instrumentation/*methods
PMC - PMC2871726
EDAT- 2010/01/23 06:00
MHDA- 2010/07/08 06:00
PMCR- 2011/03/01
CRDT- 2010/01/23 06:00
PHST- 2010/01/23 06:00 [entrez]
PHST- 2010/01/23 06:00 [pubmed]
PHST- 2010/07/08 06:00 [medline]
PHST- 2011/03/01 00:00 [pmc-release]
AID - S1525-1578(10)60048-7 [pii]
AID - JMDI60048 [pii]
AID - 10.2353/jmoldx.2010.090046 [doi]
PST - ppublish
SO  - J Mol Diagn. 2010 Mar;12(2):197-203. doi: 10.2353/jmoldx.2010.090046. Epub 2010 
      Jan 21.