PMID- 2009372
OWN - NLM
STAT- MEDLINE
DCOM- 19910506
LR  - 20210216
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 77
IP  - 7
DP  - 1991 Apr 1
TI  - Oncogene rearrangements in chronic B-cell leukemia.
PG  - 1560-4
AB  - Forty-four B-chronic lymphocytic leukemias (CLL) were studied by Southern blot 
      analysis using probes for the Ig genes and bcl-1, bcl-2 (major, minor and 5' 
      breakpoint region), bcl-3, c-myc, and retinoblastoma (Rb) loci. Eight cases had 
      three or more rearranged JH bands, indicating oligoclonality, clonal evolution, 
      or chromosomal translocation. One case had a rearrangement of the bcl-1 locus and 
      three of the bcl-2 locus. In the first case, comigration of the rearranged bcl-1 
      and JH sequences indicated a t(11;14)(q13;q32) translocation, which, in contrast 
      to previously described cases, seems to be completely reciprocal. One case with a 
      bcl-2 rearrangement showed comigration of the bcl-2 major breakpoint region and a 
      rearranged JH band. This indicates a t(14;18) (q32;q21). The two other cases 
      showed rearrangements of the bcl-2 5' breakpoint region without apparent 
      comigration. No rearrangements were detected of c-myc and bcl-3, located at 
      chromosome 19, nor was a deletion of Rb found. All but three cases had CD5 
      expression. The exceptions included the t(11;14) and the t(14;18) cases. Our 
      results confirm recent data on rearrangements at the 5' site of bcl-2 in CLL. 
      Additionally, they corroborate the presumption that CD5-negative chronic B-cell 
      leukemias should be considered apart from classical CLL.
FAU - Raghoebier, S
AU  - Raghoebier S
AD  - Department of Pathology, University of Leiden, The Netherlands.
FAU - van Krieken, J H
AU  - van Krieken JH
FAU - Kluin-Nelemans, J C
AU  - Kluin-Nelemans JC
FAU - Gillis, A
AU  - Gillis A
FAU - van Ommen, G J
AU  - van Ommen GJ
FAU - Ginsberg, A M
AU  - Ginsberg AM
FAU - Raffeld, M
AU  - Raffeld M
FAU - Kluin, P M
AU  - Kluin PM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antigens, CD)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Antigens, CD/genetics
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - DNA, Neoplasm/blood/genetics/isolation & purification
MH  - *Gene Rearrangement
MH  - *Genes, Immunoglobulin
MH  - Genes, Retinoblastoma
MH  - Genotype
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/blood/*genetics/immunology
MH  - *Oncogenes
MH  - Phenotype
MH  - Restriction Mapping
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - S0006-4971(20)83296-0 [pii]
PST - ppublish
SO  - Blood. 1991 Apr 1;77(7):1560-4.