PMID- 20095802
OWN - NLM
STAT- MEDLINE
DCOM- 20101207
LR  - 20231213
IS  - 1532-2513 (Electronic)
IS  - 0892-3973 (Linking)
VI  - 32
IP  - 3
DP  - 2010 Sep
TI  - Aqueous extract of Phyllanthus niruri (Euphorbiaceae) enhances the phenotypic and 
      functional maturation of bone marrow-derived dendritic cells and their 
      antigen-presentation function.
PG  - 393-401
LID - 10.3109/08923970903463939 [doi]
AB  - Decoctions of Phyllanthus niruri (PN) (Fam. Euphorbiaceae) is promoted in 
      traditional medicine of Africa, Asia, and South America as beneficial supplement 
      for different infectious diseases, especially for viral hepatitis, tumor, and for 
      immune compromised patients. This stimulated the interest in understanding the 
      mechanisms by which the whole extract of the plant could stimulate the immune 
      system. Dendritic cells (DCs) are professional antigen-presenting cells and 
      provide a link between the innate and the adaptive immune responses. In the 
      present study, the effects of lyophilized aqueous extract of PN on structural and 
      functional maturation of murine bone marrow-derived DCs (BM-DCs) were 
      investigated. Bone marrow cells were cultured in the presence of granulocyte 
      macrophage-colony stimulating factor and interleukin-4 (IL-4) and the generated 
      immature DCs were stimulated with PN (25, 50, and 100 microg/mL) or 
      lipopolysaccharide (10 microg/mL) for 48 h. Results showed that treatment with PN 
      increased the expression of major histocompatibility complex-II and the various 
      makers for DCs maturation (CD40), activation (CD83), and costimulation (CD86) in 
      a concentration-dependent manner. Consistent with the increase in phenotypic 
      makers, functional maturation assay showed that treatment of BM-DCs with PN 
      caused a decrease in fluorescein isothiocyanate-dextran pinocytosis and an 
      increase in IL-12 in the supernatant. In a transgenic T-cell activation model, 
      PN-treated BM-DCs presented Ova antigen to Ova-specific CD8(+) T cells from OT-1 
      mice more efficiently as demonstrated by increased T-cells proliferation and IL-2 
      production. Therefore, PN enhances the structural and functional maturation of 
      BM-DCs and their antigen-presenting function. These effects are relevant in 
      immunodeficient conditions, tumor control, and in infectious diseases.
FAU - Nworu, Chukwuemeka S
AU  - Nworu CS
AD  - Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, 
      University of Nigeria, Nsukka, Nigeria. csnworu@yahoo.com
FAU - Akah, Peter A
AU  - Akah PA
FAU - Okoye, Festus B C
AU  - Okoye FB
FAU - Esimone, Charles O
AU  - Esimone CO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunopharmacol Immunotoxicol
JT  - Immunopharmacology and immunotoxicology
JID - 8800150
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Immunoglobulins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Plant Extracts)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*drug effects/immunology
MH  - Antigens, CD/analysis/immunology
MH  - B7-2 Antigen/analysis/immunology
MH  - CD40 Antigens/analysis/immunology
MH  - CD8-Positive T-Lymphocytes/drug effects/immunology
MH  - Cell Differentiation/*drug effects/immunology
MH  - Dendritic Cells/*drug effects/immunology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/immunology
MH  - Immunoglobulins/analysis/immunology
MH  - Interleukin-12/analysis/immunology
MH  - Interleukin-4/immunology
MH  - Lipopolysaccharides/immunology
MH  - Major Histocompatibility Complex/drug effects/immunology
MH  - Membrane Glycoproteins/analysis/immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - *Phyllanthus
MH  - Pinocytosis/drug effects/immunology
MH  - Plant Extracts/immunology/*pharmacology
MH  - CD83 Antigen
EDAT- 2010/01/26 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/01/26 06:00
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.3109/08923970903463939 [doi]
PST - ppublish
SO  - Immunopharmacol Immunotoxicol. 2010 Sep;32(3):393-401. doi: 
      10.3109/08923970903463939.