PMID- 20096071
OWN - NLM
STAT- MEDLINE
DCOM- 20100204
LR  - 20220309
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Print)
IS  - 0959-9673 (Linking)
VI  - 91
IP  - 1
DP  - 2010 Feb
TI  - Oxidized low density lipoprotein-induced transdifferentiation of bone 
      marrow-derived smooth muscle-like cells into foam-like cells in vitro.
PG  - 24-33
LID - 10.1111/j.1365-2613.2009.00693.x [doi]
AB  - Oxidized-low density lipoprotein (ox-LDL) is believed to contribute to 
      atherogenesis in part by being taken up into smooth muscle cells (SMC) via 
      specific scavenger receptors; however, it is not clear whether ox-LDL receptor(s) 
      are expressed in bone marrow-derived smooth muscle-like cells (SMLCs) and whether 
      they play a role in the process of SMLC development. Therefore, we examined the 
      ox-LDL-induced transdifferentiation of SMLCs that is mediated by lectin-like 
      ox-LDL receptor-1 (LOX-1). Smooth muscle progenitor cells (SMPCs) from bone 
      marrow mesenchymal stem cells (BMSCs) were isolated using a tissue-specific 
      promoter sorting method with a mouse SM22_ promoter (_480 bp)/green fluorescent 
      protein recombinant plasmid. The SMPCs were myocardin+CD105+KDR+CD45(-)CD34(-), 
      but did not express SMC-specific markers alpha-smooth muscle actin (alpha-SMA), 
      SM22, smooth muscle myosin heavy chain (SM-MHC) and smoothelin. After long-term 
      culture with platelet-derived growth factor-BB (PDGF-BB), SMPCs expressed 
      alpha-SMA, SM22 and SM-MHC and differentiated into SMLCs. When SMLCs were 
      incubated with different concentrations of ox-LDL, LOX-1 expression on the 
      surface of SMLCs gradually increased with the increase of the ox-LDL 
      concentration, but myocardin and SMC-specific marker genes decreased, 
      accordingly. Furthermore, receptor-mediated endocytosis was enhanced and lipid 
      droplets accumulated in the cytoplasm of SMLCs. A subpopulation of 
      myocardin+CD105+KDR+CD45(-)CD34(-) SMPCs exist in BMSCs that can differentiate 
      into SMLCs under induction with PDGF-BB. Moreover, LOX-1 contributes to the 
      ox-LDL-induced transdifferentiation of bone marrow-derived SMLCs into foam-like 
      cells.
FAU - Yu, Jun
AU  - Yu J
AD  - Institute of Pathology, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, China.
FAU - Li, Yan
AU  - Li Y
FAU - Li, Mincai
AU  - Li M
FAU - Qu, Zhiling
AU  - Qu Z
FAU - Ruan, Qiurong
AU  - Ruan Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
RN  - 0 (Biomarkers)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Olr1 protein, mouse)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (Scavenger Receptors, Class E)
RN  - 0 (Tagln protein, mouse)
RN  - 0 (oxidized low density lipoprotein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 1B56C968OA (Becaplermin)
SB  - IM
MH  - Animals
MH  - Becaplermin
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/immunology/*metabolism
MH  - Cell Separation/methods
MH  - Cell Shape
MH  - *Cell Transdifferentiation
MH  - Cells, Cultured
MH  - Endocytosis
MH  - Flow Cytometry
MH  - Foam Cells/immunology/*metabolism
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - Lipid Metabolism
MH  - Lipoproteins, LDL/*metabolism
MH  - Mesenchymal Stem Cells/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microfilament Proteins/genetics
MH  - Muscle Proteins/genetics
MH  - Myocytes, Smooth Muscle/immunology/*metabolism
MH  - Phenotype
MH  - Platelet-Derived Growth Factor/metabolism
MH  - Promoter Regions, Genetic
MH  - Proto-Oncogene Proteins c-sis
MH  - Scavenger Receptors, Class E/metabolism
MH  - Time Factors
MH  - Transfection
PMC - PMC2812725
EDAT- 2010/01/26 06:00
MHDA- 2010/02/05 06:00
PMCR- 2011/02/01
CRDT- 2010/01/26 06:00
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/02/05 06:00 [medline]
PHST- 2011/02/01 00:00 [pmc-release]
AID - IEP693 [pii]
AID - 10.1111/j.1365-2613.2009.00693.x [doi]
PST - ppublish
SO  - Int J Exp Pathol. 2010 Feb;91(1):24-33. doi: 10.1111/j.1365-2613.2009.00693.x.