PMID- 20097055
OWN - NLM
STAT- MEDLINE
DCOM- 20100517
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 25
IP  - 7
DP  - 2010 Mar 15
TI  - Simplified calibration and analysis on screen-printed disposable platforms for 
      electrochemical magnetic bead-based immunosensing of zearalenone in baby food 
      samples.
PG  - 1755-60
LID - 10.1016/j.bios.2009.12.031 [doi]
AB  - An electrochemical immunosensor involving magnetic beads and disposable carbon 
      screen-printed electrodes (SPEs) for zearalenone (Zea) sensing has been developed 
      and evaluated using a certified reference material (CRM) and selected baby food 
      samples. After the immunochemical reaction, the modified paramagnetic beads were 
      confined by a magnet on surface of SPE platforms where electrochemical detection 
      is simply achieved through the suitable substrate and mediator for the HRP 
      enzyme. A remarkable detection limit of 0.007 microg L(-1) and excellent accuracy 
      with recovery rate of 101-111% showed the proposed system to be a very suitable 
      screening tool for the analysis of zearalenone in baby food samples. A new 
      simple, fast and reliable strategy involving the sequential performing of 
      calibration and analysis of target mycotoxin using just one disposable SPE each 
      is additionally proposed. Excellent analytical performance in terms of accuracy 
      and precision were again obtained with a remarkable low systematic error (less 
      than 4%) and excellent reproducibility (RSD=6%). This strategy enhanced the 
      analytical merits of immunosensor approach towards truly analytical disposable 
      tools for food-safety scene.
CI  - (c) 2009 Elsevier B.V. All rights reserved.
FAU - Hervas, Mirian
AU  - Hervas M
AD  - Departamento Quimica Analitica, Universidad de Alcala, Ctra. Madrid-Barcelona, 
      Km. 33,600, E-28871 Alcala de Henares, Madrid, Spain.
FAU - Lopez, Miguel Angel
AU  - Lopez MA
FAU - Escarpa, Alberto
AU  - Escarpa A
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091229
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Biosensing Techniques/*instrumentation/methods
MH  - Calibration
MH  - Conductometry/*instrumentation/methods
MH  - *Disposable Equipment
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Food Analysis/*instrumentation/standards
MH  - Food Contamination/*analysis
MH  - Immunomagnetic Separation/*instrumentation/standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spain
MH  - Zearalenone/*analysis/chemistry
EDAT- 2010/01/26 06:00
MHDA- 2010/05/18 06:00
CRDT- 2010/01/26 06:00
PHST- 2009/12/02 00:00 [received]
PHST- 2009/12/21 00:00 [accepted]
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/05/18 06:00 [medline]
AID - S0956-5663(09)00709-X [pii]
AID - 10.1016/j.bios.2009.12.031 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2010 Mar 15;25(7):1755-60. doi: 10.1016/j.bios.2009.12.031. 
      Epub 2009 Dec 29.