PMID- 20097242
OWN - NLM
STAT- MEDLINE
DCOM- 20100903
LR  - 20111117
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 4
DP  - 2010 Apr
TI  - Human leukocyte antigen-G molecules are constitutively expressed by synovial 
      fibroblasts and upmodulated in osteoarthritis.
PG  - 342-50
LID - 10.1016/j.humimm.2010.01.015 [doi]
AB  - Human leukocyte antigen (HLA)-G molecules are nonclassical HLA class I antigens 
      expressed as membrane bound and soluble isoforms (sHLA-G) with a restricted 
      tissue distribution and anti-inflammatory functions. Because inflammation is 
      involved in the pathogenesis of osteoarthritis (OA), we have analyzed the 
      expression and production of HLA-G molecules in in vitro cultured synovial 
      fibroblasts (SFs) from OA patients and control subjects. We have analyzed the 
      levels of sHLA-G1 and HLA-G5 isoforms by immunoenzymatic assay (enzyme-linked 
      immunosorbent assay) in the SF culture supernatants from six OA patients and six 
      control subjects in 70-day in vitro cultures and after the addition of 
      lipopolysaccharide or recombinant interleukin (IL)-10 (rIL-10). We have confirmed 
      HLA-G modulation by cytofluorimetry and immunofluorescence. The results have 
      demonstrated the spontaneous production of sHLA-G1 molecules by both OA and 
      control SFs. The expression was confirmed by cytofluorimetry and 
      immunofluorescence. OA SFs produce both sHLA-G1 and HLA-G5 molecules during the 
      first 23 days of culture and higher levels of sHLA-G1 during the first 40 days of 
      in vitro culture and after lipopolysaccharide or rIL-10 activation compared with 
      control SFs. The production of HLA-G1 molecules, constitutively expressed by 
      control and OA SFs, is significantly increased in OA, suggesting a possible 
      mechanism to counteract the inflammation of the synovial joints.
FAU - Ongaro, Alessia
AU  - Ongaro A
AD  - Department of Morphology and Embryology, University of Ferrara, Ferrara, Italy.
FAU - Stignani, Marina
AU  - Stignani M
FAU - Pellati, Agnese
AU  - Pellati A
FAU - Melchiorri, Loredana
AU  - Melchiorri L
FAU - Massari, Leo
AU  - Massari L
FAU - Caruso, Gaetano
AU  - Caruso G
FAU - De Mattei, Monica
AU  - De Mattei M
FAU - Caruso, Angelo
AU  - Caruso A
FAU - Baricordi, Olavio R
AU  - Baricordi OR
FAU - Rizzo, Roberta
AU  - Rizzo R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100206
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Lipopolysaccharides)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Separation
MH  - Cells, Cultured
MH  - Female
MH  - Fibroblasts/immunology/*metabolism/pathology
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Gene Expression Regulation/immunology
MH  - HLA Antigens/genetics/immunology/*metabolism
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/genetics/immunology/*metabolism
MH  - Humans
MH  - Interleukin-10/immunology/metabolism
MH  - Lipopolysaccharides/immunology/metabolism
MH  - Male
MH  - Middle Aged
MH  - Osteoarthritis/genetics/*immunology/metabolism/pathology
MH  - Synovial Membrane/*pathology
EDAT- 2010/01/26 06:00
MHDA- 2010/09/04 06:00
CRDT- 2010/01/26 06:00
PHST- 2009/07/30 00:00 [received]
PHST- 2009/12/15 00:00 [revised]
PHST- 2010/01/15 00:00 [accepted]
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/09/04 06:00 [medline]
AID - S0198-8859(10)00025-X [pii]
AID - 10.1016/j.humimm.2010.01.015 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Apr;71(4):342-50. doi: 10.1016/j.humimm.2010.01.015. Epub 2010 
      Feb 6.