PMID- 20097828
OWN - NLM
STAT- MEDLINE
DCOM- 20100518
LR  - 20220321
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 76
IP  - 6
DP  - 2010 Mar
TI  - Anaerobic digestion of renewable biomass: thermophilic temperature governs 
      methanogen population dynamics.
PG  - 1842-50
LID - 10.1128/AEM.02397-09 [doi]
AB  - Beet silage and beet juice were digested continuously as representative energy 
      crops in a thermophilic biogas fermentor for more than 7 years. Fluorescence 
      microscopy of 15 samples covering a period of 650 days revealed that a decrease 
      in temperature from 60 degrees C to 55 degrees C converted a morphologically 
      uniform archaeal population (rods) into a population of methanogens exhibiting 
      different cellular morphologies (rods and coccoid cells). A subsequent 
      temperature increase back to 60 degrees C reestablished the uniform morphology of 
      methanogens observed in the previous 60 degrees C period. In order to verify 
      these observations, representative samples were investigated by amplified rRNA 
      gene restriction analysis (ARDRA) and fluorescence in situ hybridization (FISH). 
      Both methods confirmed the temperature-dependent population shift observed by 
      fluorescence microscopy. Moreover, all samples investigated demonstrated that 
      hydrogenotrophic Methanobacteriales dominated in the fermentor, as 29 of 34 
      identified operational taxonomic units (OTUs) were assigned to this order. This 
      apparent discrimination of acetoclastic methanogens contradicts common models for 
      anaerobic digestion processes, such as anaerobic digestion model 1 (ADM1), which 
      describes the acetotrophic Euryarchaeota as predominant organisms.
FAU - Krakat, Niclas
AU  - Krakat N
AD  - Hamburg University of Applied Sciences, Research Centre of Lifetec Process 
      Engineering, Lohbrugger Kirchstr. 65, 21033 Hamburg-Bergedorf, Germany. 
      niclas.krakat@haw-hamburg.de
FAU - Westphal, A
AU  - Westphal A
FAU - Schmidt, S
AU  - Schmidt S
FAU - Scherer, P
AU  - Scherer P
LA  - eng
SI  - GENBANK/FN547929
SI  - GENBANK/FN547962
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100122
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Anaerobiosis
MH  - Archaea/classification/*growth & development/metabolism
MH  - Bacteria/classification/*growth & development/metabolism
MH  - Beta vulgaris/*metabolism
MH  - Bioreactors/*microbiology
MH  - DNA Fingerprinting
MH  - In Situ Hybridization, Fluorescence
MH  - Metagenomics
MH  - Methane/*metabolism
MH  - Molecular Sequence Data
MH  - Sequence Analysis, DNA
MH  - Silage/*microbiology
MH  - *Temperature
PMC - PMC2838025
EDAT- 2010/01/26 06:00
MHDA- 2010/05/19 06:00
PMCR- 2010/09/01
CRDT- 2010/01/26 06:00
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/05/19 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - AEM.02397-09 [pii]
AID - 2397-09 [pii]
AID - 10.1128/AEM.02397-09 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2010 Mar;76(6):1842-50. doi: 10.1128/AEM.02397-09. Epub 
      2010 Jan 22.