PMID- 20098355
OWN - NLM
STAT- MEDLINE
DCOM- 20100429
LR  - 20151119
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 89
IP  - 8
DP  - 2010 Apr 27
TI  - Activation of adventitial fibroblasts in the early stage of the aortic transplant 
      vasculopathy in rat.
PG  - 945-53
LID - 10.1097/TP.0b013e3181d05aa7 [doi]
AB  - BACKGROUND: Transplant vasculopathy (TV) is the most significant obstacle to 
      long-term success of organ transplantation. Increasing attention has been paid to 
      the role of adventitia in vascular diseases. We evaluated the role of adventitial 
      fibroblasts in the development of TV. METHODS: Thoracic aortas from 
      Sprague-Dawley (SD) rats transplanted into the abdominal aortas of Wistar rats 
      worked as allografts, and isografts (SD to SD) were control. Grafts were removed 
      on days 3, 7, and 14 for histologic, morphometric, and immunohistochemical 
      detection of vimentin, alpha-smooth muscle actin, Ki-67, CD3, transforming growth 
      factor-beta1 (TGF-beta1), monocyte chemoattractant protein-1 (MCP-1), matrix 
      metalloproteinase-7 (MMP-7), and quantitative real-time reverse transcriptase 
      polymerase chain reaction for TGF-beta1, MCP-1, MMP-7, tumor necrosis 
      factor-alpha, and interleukin-1beta. RESULTS: In the allografts, neointima 
      thickness and neointima/media thickness ratios were slightly increased at 7 days 
      and significantly increased at 14 days. Immunostaining of vimentin and 
      alpha-smooth muscle actin showed adventitial fibroblasts activation and 
      differentiation into myofibroblasts. Ki-67-positive nuclei were observed in the 
      adventitia 3 days after allografting and subsequently in the neointima. No more 
      than 4% CD3-positive cells were found in adventitia in all the groups. Compared 
      with isografts, TGF-beta1, MMP-7, and MCP-1 were expressed in the adventitia 
      before neointima formation and were significantly increased in allografts at all 
      time points. Tumor necrosis factor-alpha and interleukin-1beta were also 
      significantly increased in adventitia in allografts. CONCLUSIONS: These results 
      demonstrated that adventitial fibroblasts are activated and can produce cytokines 
      and chemokines before the neointimal hyperplasia. They may exert a potential 
      effect on the development of neointimal hyperplasia in TV.
FAU - Ji, Jian
AU  - Ji J
AD  - Department of Pathophysiology, School of Medicine, Shandong University, Jinan, 
      Shandong, China.
FAU - Xu, Fang
AU  - Xu F
FAU - Li, Li
AU  - Li L
FAU - Chen, Rong
AU  - Chen R
FAU - Wang, Jianli
AU  - Wang J
FAU - Hu, Wei-cheng
AU  - Hu WC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Biomarkers)
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Aorta, Abdominal/immunology/metabolism/pathology/*surgery
MH  - Aorta, Thoracic/*transplantation
MH  - Aortitis/immunology/metabolism/*pathology
MH  - Biomarkers/metabolism
MH  - Cell Proliferation
MH  - Chemokines/genetics
MH  - Connective Tissue/immunology/metabolism/*pathology
MH  - Cytokines/genetics
MH  - Disease Models, Animal
MH  - Fibroblasts/immunology/metabolism/*pathology
MH  - Hyperplasia
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - Phenotype
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
MH  - Time Factors
MH  - Transplantation, Homologous
MH  - Transplantation, Isogeneic
MH  - Tunica Intima/pathology
EDAT- 2010/01/26 06:00
MHDA- 2010/04/30 06:00
CRDT- 2010/01/26 06:00
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/04/30 06:00 [medline]
AID - 10.1097/TP.0b013e3181d05aa7 [doi]
PST - ppublish
SO  - Transplantation. 2010 Apr 27;89(8):945-53. doi: 10.1097/TP.0b013e3181d05aa7.