PMID- 20099312
OWN - NLM
STAT- MEDLINE
DCOM- 20100406
LR  - 20191210
IS  - 1934-662X (Print)
IS  - 1934-662X (Linking)
VI  - 118
IP  - 1
DP  - 2010 Feb 25
TI  - "FISHing" to detect urinary and other cancers: validation of an imaging system to 
      aid in interpretation.
PG  - 56-64
LID - 10.1002/cncy.20066 [doi]
AB  - BACKGROUND: The UroVysion Bladder Cancer Kit detects amplifications of 
      chromosomes 3, 7, and 17, and the deletion of 9p21, by fluorescence in situ 
      hybridization (FISH). Because manual interpretation of UroVysion FISH is time 
      consuming and can be challenged by variable probe signal strengths and background 
      labeling, the authors investigated an automated image analysis system to improve 
      throughput, productivity, quality control, and accuracy. METHODS: The authors 
      evaluated the interactive BioView Duet imaging system as an aid to UroVysion FISH 
      interpretation in a 2-armed, blinded comparison with manual screens of the same 
      135 consecutive cases. Manual and Duet-assisted interpretations were compared 
      with respect to concordance, reproducibility, and timing. RESULTS: Eighty-one 
      cases were interpreted as positive or negative with 94% concordance and a kappa 
      value of 0.84 between manual and Duet-aided interpretations. Three cases that 
      ultimately were judged positive were detected with the aid of Duet but were 
      missed with a manual screen. A final interpretation could not be given for 
      approximately 25% of Duet-scanned cases. Duet-aided interpretation was highly 
      reproducible for patient and control slides. Pathologist evaluation time per case 
      was 4 minutes compared with 30 minutes for manual interpretation. 
      Cytotechnologist involvement added 18 minutes for a total of 22 minutes, a 
      savings of 8 minutes per case. CONCLUSIONS: Duet-aided interpretations were at 
      least equivalent to manual interpretations. The system permitted interactive 
      review of abnormal cells and had the ability to evaluate the same cells for 
      brightfield cytology followed by FISH. The image processing and analysis tools of 
      the Duet system enhanced the morphology skills of cytology professionals in 
      providing accurate interpretations.
CI  - (c) 2010 American Cancer Society.
FAU - Smith, G Denice
AU  - Smith GD
AD  - Associated Regional and University Pathologists (ARUP) Laboratories Institute for 
      Clinical and Experimental Pathology, ARUP Laboratories, Inc., Salt Lake City, 
      Utah, USA.
FAU - Bentz, Joel S
AU  - Bentz JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Cancer Cytopathol
JT  - Cancer cytopathology
JID - 101499453
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cytological Techniques
MH  - *Diagnostic Imaging
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - *Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - Single-Blind Method
MH  - Urinary Bladder Neoplasms/*diagnosis/genetics/urine
MH  - Urologic Neoplasms/*diagnosis/genetics/urine
MH  - Vaginal Smears
MH  - Young Adult
EDAT- 2010/01/26 06:00
MHDA- 2010/04/07 06:00
CRDT- 2010/01/26 06:00
PHST- 2010/01/26 06:00 [entrez]
PHST- 2010/01/26 06:00 [pubmed]
PHST- 2010/04/07 06:00 [medline]
AID - 10.1002/cncy.20066 [doi]
PST - ppublish
SO  - Cancer Cytopathol. 2010 Feb 25;118(1):56-64. doi: 10.1002/cncy.20066.