PMID- 2009947
OWN - NLM
STAT- MEDLINE
DCOM- 19910508
LR  - 20190907
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 46
IP  - 3
DP  - 1991 Mar
TI  - Transforming growth factor beta expression in human marrow stromal cells.
PG  - 140-5
AB  - The effects of hematopoietic cytokines on the expression of transforming growth 
      factors (TGF beta) mRNA and the effect of TGF beta on cytokine and on a major 
      extracellular matrix protein, collagen I, mRNA expression was studied in human 
      marrow stromal cells. As with other cultured mesenchymal cells, stromal cells 
      constitutively express TGF beta 1 but not TGF alpha mRNA. In simian virus 40 
      (SV40)-transformed stromal cells downregulation of TGF beta 1 expression was 
      observed 2 hours after incubation with recombinant human (rh) tumor-necrosis 
      factor alpha (TNF alpha) and 144 h after addition of rh granulocyte macrophage 
      colony-stimulating factor (GM-CSF). Neither interleukin-1 (IL-1)beta nor IL-6 had 
      an observable effect on TGF beta 1 mRNA expression. TGF beta upregulated collagen 
      I mRNA expression. These data suggest that cytokines may influence TGF beta mRNA 
      expression.
FAU - Nemunaitis, J
AU  - Nemunaitis J
AD  - Medical Service, VA Medical Center, Seattle, WA 98108.
FAU - Tompkins, C K
AU  - Tompkins CK
FAU - Andrews, D F
AU  - Andrews DF
FAU - Singer, J W
AU  - Singer JW
LA  - eng
GR  - CA10333/CA/NCI NIH HHS/United States
GR  - CA18029/CA/NCI NIH HHS/United States
GR  - HL31782/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 0 (Transforming Growth Factor beta)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Blotting, Northern
MH  - Bone Marrow/*metabolism
MH  - Cells, Cultured
MH  - Collagen/biosynthesis
MH  - Gene Expression Regulation
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-1/pharmacology
MH  - Interleukin-6/pharmacology
MH  - Protein Biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Transformation, Genetic
MH  - Transforming Growth Factor alpha/pharmacology
MH  - Transforming Growth Factor beta/*genetics
MH  - Up-Regulation
EDAT- 1991/03/01 00:00
MHDA- 1991/03/01 00:01
CRDT- 1991/03/01 00:00
PHST- 1991/03/01 00:00 [pubmed]
PHST- 1991/03/01 00:01 [medline]
PHST- 1991/03/01 00:00 [entrez]
AID - 10.1111/j.1600-0609.1991.tb01267.x [doi]
PST - ppublish
SO  - Eur J Haematol. 1991 Mar;46(3):140-5. doi: 10.1111/j.1600-0609.1991.tb01267.x.