PMID- 20099842
OWN - NLM
STAT- MEDLINE
DCOM- 20100726
LR  - 20131121
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 11
IP  - 3
DP  - 2010 Mar 8
TI  - Interaction between the natural lipopeptide [Glu1, Asp5)] surfactin-C15 and 
      hemoglobin in aqueous solution.
PG  - 593-9
LID - 10.1021/bm9011453 [doi]
AB  - The interaction between natural lipopeptide [Glu(1), Asp(5)] surfactin-C15 
      (surfactin) and hemoglobin (Hb) has been studied. Surface tension measurements 
      show that the critical micelle concentration (cmc) of surfactin increases from 
      1.54 x 10(-5) to 3.86 x 10(-5) mol/L with Hb. The UV spectra display that the 
      effect of surfactin on Hb exhibits strong concentration-dependent fashion and the 
      aquometHb convert to hemichrome at high surfactin concentration. Small-angle 
      neutron scattering (SANS) and freeze-fracture transmission electron microscopy 
      (FF-TEM) measurements show that surfactin result in the formation of a fractal 
      structure representing a "necklace model" of micelle-like clusters randomly 
      distributed along the protein polypeptide chain at high surfactin concentration. 
      Far-UV circular dichroism (CD) results confirmed that surfactin can disrupt the 
      helical structure of protein at high concentrations, although the enhanced 
      native-like behavior of protein by low concentration of surfactin was observed. 
      The microenvironment change around Phe amino residues and disulfide bonds of Hb 
      was obtained from near-UV CD spectra.
FAU - Zou, Aihua
AU  - Zou A
AD  - State Key Laboratory of Bioreactor Engineering and Institute of Applied 
      Chemistry, East China University of Science and Technology, Shanghai 200237, PR 
      China.
FAU - Liu, Jing
AU  - Liu J
FAU - Garamus, Vasil M
AU  - Garamus VM
FAU - Zheng, Kai
AU  - Zheng K
FAU - Willumeit, Regine
AU  - Willumeit R
FAU - Mu, Bozhong
AU  - Mu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Hemoglobins)
RN  - 0 (Lipopeptides)
RN  - 0 (Solutions)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Aspartic Acid/*chemistry
MH  - Glutamic Acid/*chemistry
MH  - Hemoglobins/*chemistry
MH  - Lipopeptides/*chemistry
MH  - Solutions
EDAT- 2010/01/27 06:00
MHDA- 2010/07/27 06:00
CRDT- 2010/01/27 06:00
PHST- 2010/01/27 06:00 [entrez]
PHST- 2010/01/27 06:00 [pubmed]
PHST- 2010/07/27 06:00 [medline]
AID - 10.1021/bm9011453 [doi]
PST - ppublish
SO  - Biomacromolecules. 2010 Mar 8;11(3):593-9. doi: 10.1021/bm9011453.