PMID- 20100177
OWN - NLM
STAT- MEDLINE
DCOM- 20100331
LR  - 20220309
IS  - 1600-0560 (Electronic)
IS  - 0303-6987 (Linking)
VI  - 37
IP  - 2
DP  - 2010 Feb
TI  - Distinction of conjunctival melanocytic nevi from melanomas by fluorescence in 
      situ hybridization.
PG  - 196-203
LID - 10.1111/j.1600-0560.2009.01488.x [doi]
AB  - A conjunctival melanocytic nevus may on occasion be difficult to distinguish from 
      melanoma both clinically and histopathologically. An unambiguous correct 
      diagnosis is critical because of major differences in management and prognosis. 
      We evaluated a fluorescence in situ hybridization (FISH) assay, which has 
      previously been shown to be of value for the diagnosis of melanocytic nevi and 
      melanomas of the skin, using probes targeting 6p25 (RREB1), 6q23 (MYB), 11q13 
      (CCND1) and centromere 6 (CEP6), for its potential to assist in the distinction 
      of conjunctival melanocytic nevi from melanomas. Four melanocytic nevi and eight 
      melanomas of the conjunctiva were analyzed. Two of the melanomas were 
      diagnostically problematic because of suboptimal histopathology. None of the 
      conjunctival melanocytic nevi showed a level of chromosomal aberrations that met 
      FISH criteria for a diagnosis of melanoma. All eight conjunctival melanomas (six 
      unequivocal and two suspicious lesions) met FISH criteria for melanoma. Thus, 
      results from FISH assay targeting 6p25, 6q23, 11q13 and centromere 6 correlated 
      well with the histopathologic diagnoses and supported the histopathologic 
      suspicion in two problem cases. The findings encourage further exploration of 
      this technique as an ancillary method for the work-up of conjunctival melanocytic 
      proliferations.
FAU - Busam, Klaus J
AU  - Busam KJ
AD  - Department of Pathology, Ophthalmologic Oncology Service, Memorial 
      Sloan-Kettering Cancer Center, New York, NY 10065, USA. busamk@mskcc.org
FAU - Fang, Yuqiang
AU  - Fang Y
FAU - Jhanwar, Suresh C
AU  - Jhanwar SC
FAU - Pulitzer, Melissa P
AU  - Pulitzer MP
FAU - Marr, Brian
AU  - Marr B
FAU - Abramson, David H
AU  - Abramson DH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
SB  - IM
CIN - J Cutan Pathol. 2010 Feb;37(2):193-5. PMID: 20100176
MH  - Adult
MH  - Aged
MH  - Cell Count
MH  - Child
MH  - Conjunctival Neoplasms/*diagnosis/genetics/surgery
MH  - Cryosurgery
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Melanoma/*diagnosis/genetics/surgery
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/surgery
MH  - Nevus, Pigmented/*diagnosis/genetics/surgery
EDAT- 2010/01/27 06:00
MHDA- 2010/04/01 06:00
CRDT- 2010/01/27 06:00
PHST- 2010/01/27 06:00 [entrez]
PHST- 2010/01/27 06:00 [pubmed]
PHST- 2010/04/01 06:00 [medline]
AID - CUP1488 [pii]
AID - 10.1111/j.1600-0560.2009.01488.x [doi]
PST - ppublish
SO  - J Cutan Pathol. 2010 Feb;37(2):196-203. doi: 10.1111/j.1600-0560.2009.01488.x.