PMID- 20101439
OWN - NLM
STAT- MEDLINE
DCOM- 20100910
LR  - 20211020
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 21
IP  - 5
DP  - 2010 May
TI  - Use of chitosan conduit combined with bone marrow mesenchymal stem cells for 
      promoting peripheral nerve regeneration.
PG  - 1713-20
LID - 10.1007/s10856-010-4003-y [doi]
AB  - Many studies have been dedicated to the development of scaffolds for improving 
      post-traumatic nerve regeneration. The goal of this study was to develop and test 
      chitosan conduit to use in peripheral nerve reconstruction, either alone or 
      combined with bone marrow mesenchymal stem cells (BMSCs). In this study, the 
      roles of the degree of deacetylation (DD) and molecular weight of chitosan on 
      some biological properties of chitosan films, including hydrophilicity, 
      degradation and BMSCs affinity were investigated. The molecular weight of 
      Chitosans used are 5 x 10(4) Da, 2 x 10(5) Da, 5 x 10(5) Da, 1 x 10(6) Da, the 
      deacetylation degrees are 85, 95%, respectively. The affinity of eight kinds of 
      Chitosans to the BMSCs was assessed by MTT assay, the contact angle and the 
      degradation time of the materials in vivo were also measured. Chitosans with the 
      molecular weight of 1 x 10(6) Da and DD of 95% can significantly promote the 
      survival and outgrowth of cells, which have better hydrophilicity and can remain 
      integrity even after 8 to 16 weeks, all of above meet the requirement of nerve 
      engineering. The BMSCs we transplanted can differentiate into neural stem cells 
      in vivo, and the materials we selected combined with BMSCs can bridge 8-mm-long 
      neural gap better resulting from the differentiation effects of the BMSCs.
FAU - Zheng, Lei
AU  - Zheng L
AD  - Institute of Pharmacy, Shandong Traffic Hospital, 250031, Jinan, China.
FAU - Cui, Hui-Fei
AU  - Cui HF
LA  - eng
PT  - Journal Article
DEP - 20100126
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Animals
MH  - Bone Marrow/metabolism
MH  - Cell Differentiation/drug effects
MH  - Chitosan/metabolism/*pharmacology
MH  - Hematopoietic Stem Cells/metabolism
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Nerve Regeneration/drug effects/*physiology
MH  - Peripheral Nerves/metabolism
MH  - Prostheses and Implants
MH  - Regeneration/drug effects
EDAT- 2010/01/27 06:00
MHDA- 2010/09/11 06:00
CRDT- 2010/01/27 06:00
PHST- 2009/12/11 00:00 [received]
PHST- 2010/01/11 00:00 [accepted]
PHST- 2010/01/27 06:00 [entrez]
PHST- 2010/01/27 06:00 [pubmed]
PHST- 2010/09/11 06:00 [medline]
AID - 10.1007/s10856-010-4003-y [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2010 May;21(5):1713-20. doi: 10.1007/s10856-010-4003-y. 
      Epub 2010 Jan 26.