PMID- 20101494
OWN - NLM
STAT- MEDLINE
DCOM- 20100920
LR  - 20211203
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 397
IP  - 5
DP  - 2010 Jul
TI  - Comparison of analytical tools and biological assays for detection of paralytic 
      shellfish poisoning toxins.
PG  - 1655-71
LID - 10.1007/s00216-010-3459-4 [doi]
AB  - The paralytic shellfish poisoning toxins (PSTs) were, as their name suggests, 
      discovered as a result of human poisoning after consumption of contaminated 
      shellfish. More recently, however, the same toxins have been found to be produced 
      by freshwater cyanobacteria. These organisms have worldwide distribution and are 
      common in our sources of drinking water, thus presenting another route of 
      potential human exposure. However, the regulatory limits for PSTs in drinking 
      water are considerably lower than in shellfish. This has increased the need to 
      find alternatives to the mouse bioassay, which, apart from being ethically 
      questionable, does not have a limit of detection capable of detecting the PSTs in 
      water at the regulated concentrations. Additionally, the number of naturally 
      occurring PSTs has grown substantially since saxitoxin was first characterised, 
      markedly increasing the analytical challenge of this group of compounds. This 
      paper summarises the development of chromatographic, toxicity, and molecular 
      sensor binding methodologies for detection of the PSTs in shellfish, 
      cyanobacteria, and water contaminated by these toxins. It then summarises the 
      advantages and disadvantages of their use for particular applications. Finally it 
      recommends some future requirements that will contribute to their improvement for 
      these applications.
FAU - Humpage, A R
AU  - Humpage AR
AD  - Australian Water Quality Centre, GPO Box 1751, Adelaide, SA, 5001, Australia. 
      Andrew.humpage@sawater.com.au
FAU - Magalhaes, V F
AU  - Magalhaes VF
FAU - Froscio, S M
AU  - Froscio SM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20100126
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cyanobacteria Toxins)
RN  - 0 (Marine Toxins)
RN  - 0 (Microcystins)
RN  - 35523-89-8 (Saxitoxin)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/*analysis/toxicity
MH  - Biological Assay/*methods
MH  - Chemistry Techniques, Analytical/*methods
MH  - Cyanobacteria Toxins
MH  - Humans
MH  - Marine Toxins/*analysis/toxicity
MH  - Mice
MH  - Microcystins/*analysis/toxicity
MH  - Rats
MH  - Saxitoxin/*analysis/toxicity
MH  - Shellfish Poisoning/diagnosis
RF  - 124
EDAT- 2010/01/27 06:00
MHDA- 2010/09/21 06:00
CRDT- 2010/01/27 06:00
PHST- 2009/10/30 00:00 [received]
PHST- 2010/01/04 00:00 [accepted]
PHST- 2009/12/22 00:00 [revised]
PHST- 2010/01/27 06:00 [entrez]
PHST- 2010/01/27 06:00 [pubmed]
PHST- 2010/09/21 06:00 [medline]
AID - 10.1007/s00216-010-3459-4 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2010 Jul;397(5):1655-71. doi: 10.1007/s00216-010-3459-4. Epub 
      2010 Jan 26.