PMID- 20101988
OWN - NLM
STAT- MEDLINE
DCOM- 20140925
LR  - 20161125
IS  - 1000-0593 (Print)
IS  - 1000-0593 (Linking)
VI  - 29
IP  - 11
DP  - 2009 Nov
TI  - [Fluorescence resonance energy transfer between gentamycin and water-soluble CdTe 
      QDs].
PG  - 3070-4
AB  - The water-soluble CdTe quantum dots (QDs) were prepared by using 
      mercaptopropionic acid (MPA) as stabilizer in the aqueous system. Fluorescence 
      resonance energy transfer (FRET) system was constructed between gentamycin 
      (acceptor) and water-soluble CdTe QDs (donor). The maximal emission wavelength 
      was 690 nm, and the line width of the fluorescence spectrum was very narrow (with 
      the full width at half-maximum about 10 nm) and symmetric. The transfer of 
      resonance energy from the CdTe QDs to gentamycin (GT) resulted in the 
      fluorescence quenching of the QDs, corresponding to the increase in the 
      concentration of GT. Several factors that impacted the fluorescence spectra of 
      the FRET system, such as the excitation wavelength (305-425 nm), pH(5.0-11.0), 
      ions (0-0.1 mmol x L(-1) PBS; 0-0.5 mmol x L(-1) NaCl), time (1-120 min), 
      temperature (5-50 degrees C), and concentration of GT (2-80 mg x L(-1)), were 
      investigated and refined. The linear ranges of GT concentration were 2-20 mg x 
      L(-1), r = 0.986 7. Fourier transform infrared spectroscopy (FTIR) and 
      high-performance liquid chromatography (HPLC) were used for confirming the 
      chemical construction and relative specificity, respectively. The results 
      indicated that sulfur and oxygen atoms in MPA molecules took part in coordination 
      with rich Cd2+ on the surface of the nanoparticles. Meanwhile the results also 
      demonstrated that the hydrogen bond between carboxyl of mercaptopropionic acid on 
      the surface of quantum dots and amidocyanogen of GT mainly contributes to 
      combining CdTe with GT. The combination ratio between GT and CdTe QDs is 0.35 to 
      1.0 according to HPLC. GT as an enhancement has first been applied to the 
      determination of the bovine serum albumin (BSA) labeled with CdTe QDs, and the 
      fluorescence intensity of the labeled BSA with GT is 6 times higher than the 
      control. The proposed method might offer an attractive potential for use in 
      future, because it is sensitive and rapid.
FAU - Li, Jin-Gui
AU  - Li JG
AD  - College of Veterinary Medicine of Yangzhou University, Yangzhou 225009, China. 
      ljggwy@yahoo.com.cn
FAU - Zhu, Kui
AU  - Zhu K
FAU - Xu, Fei
AU  - Xu F
FAU - Jiang, Hai-Yang
AU  - Jiang HY
FAU - Ding, Shuang-Yang
AU  - Ding SY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Guang Pu Xue Yu Guang Pu Fen Xi
JT  - Guang pu xue yu guang pu fen xi = Guang pu
JID - 9424805
RN  - 0 (Cadmium Compounds)
RN  - 0 (Gentamicins)
RN  - 059QF0KO0R (Water)
RN  - NQA0O090ZJ (Tellurium)
RN  - STG188WO13 (cadmium telluride)
SB  - IM
MH  - Cadmium Compounds/*chemistry
MH  - Chromatography, High Pressure Liquid
MH  - *Fluorescence Resonance Energy Transfer
MH  - Gentamicins/*chemistry
MH  - Quantum Dots/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Tellurium/*chemistry
MH  - Water/chemistry
EDAT- 2010/01/28 06:00
MHDA- 2014/09/26 06:00
CRDT- 2010/01/28 06:00
PHST- 2010/01/28 06:00 [entrez]
PHST- 2010/01/28 06:00 [pubmed]
PHST- 2014/09/26 06:00 [medline]
PST - ppublish
SO  - Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Nov;29(11):3070-4.