PMID- 20102397
OWN - NLM
STAT- MEDLINE
DCOM- 20100721
LR  - 20100127
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 56
IP  - 2
DP  - 2010 Jan
TI  - Microchimerism in renal allografts: clinicopathological associations according to 
      the type of chimeric cells.
PG  - 188-97
LID - 10.1111/j.1365-2559.2009.03466.x [doi]
AB  - AIMS: Recent studies have highlighted the presence of microchimerism in various 
      solid allografts. The biological significance of these chimeric cells is 
      controversial. They may be beneficial, leading to better tolerance of grafts or 
      participating in tissue repair or, in contrast, deleterious if involved in 
      chronic lesions. The aim was to assess the frequency and cellular nature of 
      microchimerism in female renal grafts of male recipients by combined fluorescence 
      in situ hybridization (FISH) for Y chromosome and immunohistochemistry and to 
      investigate associations between intragraft microchimerism and histological 
      lesions or allograft outcome. METHODS AND RESULTS: We screened 33 renal biopsy 
      specimens, including 11 with acute T-cell-mediated rejection and nine with 
      transplant glomerulopathy, from 22 male recipients transplanted with female 
      kidneys by FISH and immunohistochemistry with antibodies against smooth muscle 
      actin (mesangial cells), CD31 (endothelial cells), KL1 (epithelial cells), CD45 
      (leucocyte common antigen) and glomerular epithelial protein 1 (podocytes). 
      Tubular microchimerism was detected in 71% of the patients with a mean percentage 
      of chimeric epithelial cells of 1.4%. Glomerular microchimerism involving 
      podocytes, mesangial and endothelial cells was present with a mean number of 
      chimeric cells per glomerular section of, respectively, 0.6, 2.66 and 3.53. There 
      was an association between endothelial microchimerism and a previous episode of 
      acute T-cell-mediated rejection. CONCLUSIONS: In conclusion, microchimerism in 
      renal grafts occurs frequently, but at a low level and affects tubular cells and 
      all glomerular cell compartments in human renal allografts.
FAU - Ferlicot, Sophie
AU  - Ferlicot S
AD  - Service d'Anatomie et de Cytologie Pathologiques, Universite Paris-Sud 11, APHP 
      Hopital de Bicetre, Le Kremlin-Bicetre, France. sophie.ferlicot@bct.aphp.fr
FAU - Vernochet, Amelia
AU  - Vernochet A
FAU - Romana, Serge
AU  - Romana S
FAU - Ortin-Serrano, Monique
AU  - Ortin-Serrano M
FAU - Letierce, Alexia
AU  - Letierce A
FAU - Bregerie, Olivier
AU  - Bregerie O
FAU - Durrbach, Antoine
AU  - Durrbach A
FAU - Guettier, Catherine
AU  - Guettier C
LA  - eng
PT  - Journal Article
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
SB  - IM
MH  - Biopsy
MH  - *Chimerism
MH  - Chromosomes, Human, Y/genetics
MH  - Endothelial Cells/*metabolism/pathology
MH  - Female
MH  - Glomerulonephritis/*genetics
MH  - Graft Rejection/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Transplantation/*pathology/physiology
MH  - Kidney Tubules/*metabolism/pathology
MH  - Male
MH  - Mesangial Cells/*metabolism/pathology
MH  - Podocytes/*metabolism/pathology
MH  - Sex Factors
MH  - T-Lymphocytes
MH  - Transplantation, Homologous
EDAT- 2010/01/28 06:00
MHDA- 2010/07/22 06:00
CRDT- 2010/01/28 06:00
PHST- 2010/01/28 06:00 [entrez]
PHST- 2010/01/28 06:00 [pubmed]
PHST- 2010/07/22 06:00 [medline]
AID - HIS3466 [pii]
AID - 10.1111/j.1365-2559.2009.03466.x [doi]
PST - ppublish
SO  - Histopathology. 2010 Jan;56(2):188-97. doi: 10.1111/j.1365-2559.2009.03466.x.