PMID- 2010641
OWN - NLM
STAT- MEDLINE
DCOM- 19910506
LR  - 20190510
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 163
IP  - 4
DP  - 1991 Apr
TI  - Bioactive and inactive forms of tumor necrosis factor-alpha in spinal fluid from 
      patients with meningitis.
PG  - 886-9
AB  - Twenty-four patients, including 12 with meningitis, were admitted with meningeal 
      symptoms and fever. Their serum and spinal fluid tumor necrosis factor-alpha (TNF 
      alpha) levels were determined by ELISA. TNF alpha immunoreactivity was found in 
      spinal fluid of 10 meningitis patients and one nonmeningitis patient, whereas 7 
      sera, 5 from meningitis patients, contained TNF alpha. Levels were significantly 
      higher in spinal fluids than in serum samples, and the TNF alpha bioactivity of 
      spinal fluids and sera was considerably below predictions based on ELISA 
      measurements. Gel filtration chromatography demonstrated the presence of both 
      polymeric (greater than 200 kDa) and oligomeric (10-40 kDa) TNF alpha in spinal 
      fluid. Significant bioactivity was obtained only from samples containing 
      oligomeric cytokine. In agreement with previous in vitro findings, these results 
      strongly indicate that bioactive TNF alpha oligomers form inactive polymers and 
      monomers, which could contribute to the observed in vivo discrepancies between 
      immunoreactive and bioactive protein. Finally, the data support the concept of 
      local central nervous system production of TNF alpha in meningitis.
FAU - Moller, B
AU  - Moller B
AD  - Department of Clinical Immunology, Skejby Sygehus, University of Aarhus, Denmark.
FAU - Mogensen, S C
AU  - Mogensen SC
FAU - Wendelboe, P
AU  - Wendelboe P
FAU - Bendtzen, K
AU  - Bendtzen K
FAU - Petersen, C M
AU  - Petersen CM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Biological Assay
MH  - Child
MH  - Chromatography, Gel
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Meningitis/blood/*cerebrospinal fluid
MH  - Middle Aged
MH  - Tumor Necrosis Factor-alpha/analysis/*cerebrospinal fluid
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1093/infdis/163.4.886 [doi]
PST - ppublish
SO  - J Infect Dis. 1991 Apr;163(4):886-9. doi: 10.1093/infdis/163.4.886.