PMID- 20107535
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20211020
IS  - 2092-6685 (Electronic)
IS  - 1598-2629 (Print)
IS  - 1598-2629 (Linking)
VI  - 9
IP  - 1
DP  - 2009 Feb
TI  - Production of TGF-beta1 as a Mechanism for Defective Antigen-presenting Cell 
      Function of Macrophages Generated in vitro with M-CSF.
PG  - 27-33
LID - 10.4110/in.2009.9.1.27 [doi]
AB  - BACKGROUND: Macrophages generated in vitro using macrophage-colony stimulating 
      factor (M-CSF) and interleukin (IL)-6 from bone marrow cells (BM-Mp) are 
      defective in antigen presenting cell (APC) function as shown by their ability to 
      induce the proliferation of anti-CD3 mAb-primed syngeneic T cells. However, they 
      do express major histocompatibility (MHC) class I and II molecules, accessory 
      molecules and intracellular adhesion molecules. Here we demonstrate that the 
      defective APC function of macrophages is mainly due to production of TGF-beta1 by 
      BM-Mp. METHODS: Microarray analysis showed that TGF-beta1 was highly expressed in 
      BM-Mp, compared to a macrophage cell line, B6D, which exerted efficient APC 
      function. Production of TGF-beta1 by BM-Mp was confirmed by neutralization 
      experiments of TGF-beta1 as well as by real time-polymerase chain reaction (PCR). 
      RESULTS: Addition of anti-TGF-beta1 monoclonal antibody to cultures of BM-Mp and 
      anti-CD3 mAb-primed syngeneic T cells efficiently induced the proliferation of 
      syngeneic T cells. Conversely, the APC function of B6D cells was almost 
      completely suppressed by addition of TGF-beta1. Quantitative real time-PCR 
      analysis also confirmed the enhanced expression of TGF-beta1 in BM-Mp. 
      CONCLUSION: The defective APC function of macrophages generated in vitro with 
      M-CSF and IL-6 was mainly due to the production of TGF-beta1 by macrophages.
FAU - Lee, Jae Kwon
AU  - Lee JK
AD  - School of Science Education (Biology), Chungbuk National University, Cheongju, 
      Korea.
FAU - Lee, Young-Ran
AU  - Lee YR
FAU - Lee, Young-Hee
AU  - Lee YH
FAU - Kim, Kyungjae
AU  - Kim K
FAU - Lee, Chong-Kil
AU  - Lee CK
LA  - eng
PT  - Journal Article
DEP - 20090228
PL  - Korea (South)
TA  - Immune Netw
JT  - Immune network
JID - 101137270
PMC - PMC2803298
OTO - NOTNLM
OT  - APC function
OT  - M-CSF
OT  - TGF-beta1
OT  - macrophage
COIS- Disclosures: The authors have no financial conflict of interest.
EDAT- 2010/01/29 06:00
MHDA- 2010/01/29 06:01
PMCR- 2009/02/01
CRDT- 2010/01/29 06:00
PHST- 2008/12/09 00:00 [received]
PHST- 2008/12/19 00:00 [revised]
PHST- 2008/12/24 00:00 [accepted]
PHST- 2010/01/29 06:00 [entrez]
PHST- 2010/01/29 06:00 [pubmed]
PHST- 2010/01/29 06:01 [medline]
PHST- 2009/02/01 00:00 [pmc-release]
AID - 10.4110/in.2009.9.1.27 [doi]
PST - ppublish
SO  - Immune Netw. 2009 Feb;9(1):27-33. doi: 10.4110/in.2009.9.1.27. Epub 2009 Feb 28.