PMID- 20109033
OWN - NLM
STAT- MEDLINE
DCOM- 20110125
LR  - 20220321
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 19
IP  - 10
DP  - 2010 Oct
TI  - Islet-1 cells are cardiac progenitors present during the entire lifespan: from 
      the embryonic stage to adulthood.
PG  - 1601-15
LID - 10.1089/scd.2009.0483 [doi]
AB  - The aim of this study was to longitudinally characterize the distribution of 
      cells actively expressing the progenitor transcription factor islet-1 (Isl1+) 
      during the embryonic life, the postnatal period, and adulthood. In this study, we 
      have used direct immunohistochemical staining toward the protein Isl1 in a 
      longitudinal rat model. Cells actively expressing Isl1 were traced in embryos 
      from gestational day (GD) 11 until adulthood. In early cardiac development (GD 
      11), the Isl1+ progenitors were located in a greater abundance in the paracardiac 
      regions, areas suggested to be the second heart field. To a lesser extent, Isl1+ 
      cells were present within the bulbotruncal region and the truncus arteriosus. 
      During the following days until GD 15, the Isl1+ cells were mainly observed at 
      the proximal outflow tract (OFT) and at the inflow area of the right atrium. No 
      Isl1+ cells were detected in the left ventricle. Compared with GD 11, more Isl1+ 
      cells seemed to co-express cardiomyocyte markers and a minority of the Isl1+ 
      cells was undifferentiated. Unexpectedly, only few undifferentiated Isl1+ cells 
      were Ki67+ while a lot of TnT+ cardiomyocytes were proliferating in the 
      ventricles. After birth, immature Isl1+ cells were still present in the OFT where 
      they resided until adulthood. Our data suggest that during embryogenesis, Isl1+ 
      cells migrate from extracardiac regions into the proximal part of the heart, 
      proliferating and giving rise to cardioblasts. Unexpectedly, only a minority of 
      the Isl1+ cells while a majority of ventricular cardiomyocytes were 
      proliferating. The Isl1+ cell pool persists into adulthood, which might open up 
      new strategies to repair damaged myocardium.
FAU - Genead, Rami
AU  - Genead R
AD  - Department of Medicine, Division of Cardiology, Karolinska Institutet, Karolinska 
      University Hospital, Stockholm, Sweden.
FAU - Danielsson, Christian
AU  - Danielsson C
FAU - Andersson, Agneta B
AU  - Andersson AB
FAU - Corbascio, Matthias
AU  - Corbascio M
FAU - Franco-Cereceda, Anders
AU  - Franco-Cereceda A
FAU - Sylven, Christer
AU  - Sylven C
FAU - Grinnemo, Karl-Henrik
AU  - Grinnemo KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Biomarkers)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LIM-Homeodomain Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (insulin gene enhancer binding protein Isl-1)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cell Proliferation
MH  - Heart/anatomy & histology/*embryology/*growth & development
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - LIM-Homeodomain Proteins
MH  - Myocardium/cytology/metabolism
MH  - Myocytes, Cardiac/*cytology/metabolism
MH  - Rats
MH  - Stem Cells/cytology/*physiology
MH  - Transcription Factors
EDAT- 2010/01/30 06:00
MHDA- 2011/01/28 06:00
CRDT- 2010/01/30 06:00
PHST- 2010/01/30 06:00 [entrez]
PHST- 2010/01/30 06:00 [pubmed]
PHST- 2011/01/28 06:00 [medline]
AID - 10.1089/scd.2009.0483 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2010 Oct;19(10):1601-15. doi: 10.1089/scd.2009.0483.