PMID- 20109198
OWN - NLM
STAT- MEDLINE
DCOM- 20100503
LR  - 20211020
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 11
DP  - 2010 Jan 28
TI  - Development of genomic resources for the prairie vole (Microtus ochrogaster): 
      construction of a BAC library and vole-mouse comparative cytogenetic map.
PG  - 70
LID - 10.1186/1471-2164-11-70 [doi]
AB  - BACKGROUND: The prairie vole (Microtus ochrogaster) is a premier animal model for 
      understanding the genetic and neurological basis of social behaviors. Unlike 
      other biomedical models, prairie voles display a rich repertoire of social 
      behaviors including the formation of long-term pair bonds and biparental care. 
      However, due to a lack of genomic resources for this species, studies have been 
      limited to a handful of candidate genes. To provide a substrate for future 
      development of genomic resources for this unique model organism, we report the 
      construction and characterization of a bacterial artificial chromosome (BAC) 
      library from a single male prairie vole and a prairie vole-mouse (Mus musculus) 
      comparative cytogenetic map. RESULTS: We constructed a prairie vole BAC library 
      (CHORI-232) consisting of 194,267 recombinant clones with an average insert size 
      of 139 kb. Hybridization-based screening of the gridded library at 19 loci 
      established that the library has an average depth of coverage of approximately 
      10x. To obtain a small-scale sampling of the prairie vole genome, we generated 
      3884 BAC end-sequences totaling approximately 2.8 Mb. One-third of these BAC-end 
      sequences could be mapped to unique locations in the mouse genome, thereby 
      anchoring 1003 prairie vole BAC clones to an orthologous position in the mouse 
      genome. Fluorescence in situ hybridization (FISH) mapping of 62 prairie vole 
      clones with BAC-end sequences mapping to orthologous positions in the mouse 
      genome was used to develop a first-generation genome-wide prairie vole-mouse 
      comparative cytogenetic map. While conserved synteny was observed between this 
      pair of rodent genomes, rearrangements between the prairie vole and mouse genomes 
      were detected, including a minimum of five inversions and 16 inter-chromosomal 
      rearrangements. CONCLUSIONS: The construction of the prairie vole BAC library and 
      the vole-mouse comparative cytogenetic map represent the first genome-wide modern 
      genomic resources developed for this species. The BAC library will support future 
      genomic, genetic and molecular characterization of this genome and species, and 
      the isolation of clones of high interest to the vole research community will 
      allow for immediate characterization of the regulatory and coding sequences of 
      genes known to play important roles in social behaviors. In addition, these 
      resources provide an excellent platform for future higher resolution cytogenetic 
      mapping and full genome sequencing.
FAU - McGraw, Lisa A
AU  - McGraw LA
AD  - Center for Behavioral Neuroscience, Emory University, Atlanta, GA, USA.
FAU - Davis, Jamie K
AU  - Davis JK
FAU - Lowman, Josh J
AU  - Lowman JJ
FAU - ten Hallers, Boudewijn F H
AU  - ten Hallers BF
FAU - Koriabine, Maxim
AU  - Koriabine M
FAU - Young, Larry J
AU  - Young LJ
FAU - de Jong, Pieter J
AU  - de Jong PJ
FAU - Rudd, M Katharine
AU  - Rudd MK
FAU - Thomas, James W
AU  - Thomas JW
LA  - eng
GR  - 1F32MH079661/MH/NIMH NIH HHS/United States
GR  - 1R21MH082225/MH/NIMH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20100128
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
SB  - IM
MH  - Animals
MH  - Arvicolinae/*genetics
MH  - Cells, Cultured
MH  - Chromosomes, Artificial, Bacterial
MH  - Comparative Genomic Hybridization
MH  - *Gene Library
MH  - *Genomics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - *Physical Chromosome Mapping
MH  - Sequence Analysis, DNA
PMC - PMC2824727
EDAT- 2010/01/30 06:00
MHDA- 2010/05/04 06:00
PMCR- 2010/01/28
CRDT- 2010/01/30 06:00
PHST- 2009/11/11 00:00 [received]
PHST- 2010/01/28 00:00 [accepted]
PHST- 2010/01/30 06:00 [entrez]
PHST- 2010/01/30 06:00 [pubmed]
PHST- 2010/05/04 06:00 [medline]
PHST- 2010/01/28 00:00 [pmc-release]
AID - 1471-2164-11-70 [pii]
AID - 10.1186/1471-2164-11-70 [doi]
PST - epublish
SO  - BMC Genomics. 2010 Jan 28;11:70. doi: 10.1186/1471-2164-11-70.