PMID- 20109350
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20191111
IS  - 1000-467X (Print)
IS  - 1944-446X (Linking)
VI  - 29
IP  - 2
DP  - 2010 Feb
TI  - Minimal residual disease monitoring in chronic myeloid leukemia patients after 
      allogeneic hematopoietic stem cell transplantation using interphase fluorescence 
      in situ hybridization and real-time quantitative reverse transcription PCR.
PG  - 194-7
AB  - BACKGROUND AND OBJECTIVE: Interphase fluorescence in situ hybridization (FISH) 
      and real-time quantitative reverse transcription PCR (RQ-PCR) are the common 
      methods for monitoring minimal residual disease (MRD) in chronic myeloid leukemia 
      (CML) patients. This study was to assess the value of monitoring BCR-ABL fusion 
      gene level in CML patients after allogeneic hematopoietic stem cell 
      transplantation (allo-HSCT) using FISH and RQ-PCR. METHODS: BCR-ABL fusion gene 
      levels were detected in the bone marrow of 31 patients with CML before and 3-48 
      months after allo-HSCT using FISH and RQ-PCR. RESULTS: BCR-ABL positive cells 
      detected by FISH were decreased 3-30 months after allo-HSCT and BCR-ABL/ABL mRNA 
      was reduced by 2 logarithmic units in RQ-PCR (P < 0.05). While no BCR-ABL 
      positive cell was detected by FISH 30 months after allo-HSCT, BCR-ABL/ABL mRNA 
      was detected by RQ-PCR and declined by more than 3 logarithmic units, (P < 0.05). 
      CONCLUSIONS: Dynamic monitoring of BCR-ABL gene on molecular level in CML 
      patients after allo-HSCT is useful in the early prediction of susceptibility to 
      recurrence in the patients and in designing intervention, and is thus helpful in 
      improving the overall survival rate after transplantation.
FAU - Zhu, Hong-Qian
AU  - Zhu HQ
AD  - Department of Hematology, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510515, PR China.
FAU - Liu, Xiao-Li
AU  - Liu XL
FAU - Song, Lan-Lin
AU  - Song LL
FAU - Liu, Qi-Fa
AU  - Liu QF
FAU - Meng, Fan-Yi
AU  - Meng FY
FAU - Zhou, Shu-Yun
AU  - Zhou SY
LA  - eng
PT  - Journal Article
PL  - England
TA  - Chin J Cancer
JT  - Chinese journal of cancer
JID - 101498232
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Child
MH  - Female
MH  - Fusion Proteins, bcr-abl/genetics/*metabolism
MH  - *Hematopoietic Stem Cell Transplantation
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*metabolism/pathology/*therapy
MH  - Male
MH  - *Neoplasm, Residual
MH  - RNA, Messenger/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Young Adult
EDAT- 2010/01/30 06:00
MHDA- 2012/01/06 06:00
CRDT- 2010/01/30 06:00
PHST- 2010/01/30 06:00 [entrez]
PHST- 2010/01/30 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 1000-467X201002194 [pii]
AID - 10.5732/cjc.009.10250 [doi]
PST - ppublish
SO  - Chin J Cancer. 2010 Feb;29(2):194-7. doi: 10.5732/cjc.009.10250.