PMID- 20110680
OWN - NLM
STAT- MEDLINE
DCOM- 20100408
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 25
IP  - 2-3
DP  - 2010
TI  - Ethanol inhibits gastric acid secretion in rats through increased AMP-kinase 
      activity.
PG  - 195-202
LID - 10.1159/000276553 [doi]
AB  - The effects of ethanol on gastric acid secretion remain controversial. The 
      present study examines the effect of low-dose (2%) short term (15-20 min) ethanol 
      exposure on gastric acid secretion via a potential interaction with AMP-activated 
      protein kinase (AMPK). Real-time fluorescence digital imaging was used to provide 
      functional evidence for the interaction of ethanol and AMPK in modulating 
      secretagogue-induced acid secretion. Individual rat gastric glands were loaded 
      with the pH-sensitive dye BCECF and the secretagogues carbachol (200 microM) or 
      histamine (200 microM) were added to induce secretion. Rates of pH recovery were 
      calculated as DeltapH(i)/Deltat. In one series of experiments, 
      secretagogue-induced acid secretion was inhibited by 2% ethanol, or the AMPK 
      activator AICAR monophosphate (AICAR) (20 mM). In a separate series, 2% ethanol 
      was added in combination with compound C (20 microM), an AMPK inhibitor, to 
      prevent activation of AMPK. 2% ethanol significantly suppressed stimulated acid 
      secretion. In order to confirm modulation of AMPK activity by ethanol, the 
      specific AMPK inhibitor compound C was used, which reversed the inhibitory 
      effects of ethanol on stimulated acid secretion. This study demonstrates that low 
      dose ethanol (2%) inhibits secretagogue-dependent acid secretion by activation of 
      the AMPK pathway in rat gastric parietal cells.
CI  - Copyright 2010 S. Karger AG, Basel.
FAU - Kopic, Sascha
AU  - Kopic S
AD  - Department of Surgery, Yale University, School of Medicine, New Haven, CT 06511, 
      USA.
FAU - Corradini, Stefanie
AU  - Corradini S
FAU - Sidani, Shafik
AU  - Sidani S
FAU - Murek, Michael
AU  - Murek M
FAU - Vardanyan, Artur
AU  - Vardanyan A
FAU - Foller, Michael
AU  - Foller M
FAU - Ritter, Markus
AU  - Ritter M
FAU - Geibel, John P
AU  - Geibel JP
LA  - eng
PT  - Journal Article
DEP - 20100112
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Fluoresceins)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Ribonucleotides)
RN  - 360-97-4 (Aminoimidazole Carboxamide)
RN  - 3K9958V90M (Ethanol)
RN  - 820484N8I3 (Histamine)
RN  - 85138-49-4 (2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein)
RN  - 8Y164V895Y (Carbachol)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - F0X88YW0YK (AICA ribonucleotide)
SB  - IM
MH  - AMP-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Aminoimidazole Carboxamide/analogs & derivatives/pharmacology
MH  - Animals
MH  - Carbachol/pharmacology
MH  - Ethanol/*pharmacology
MH  - Fluoresceins/chemistry
MH  - Fluorescent Dyes/chemistry
MH  - Gastric Acid/*metabolism
MH  - Gastric Mucosa/metabolism/pathology
MH  - Histamine/pharmacology
MH  - Hydrogen-Ion Concentration
MH  - Hypoglycemic Agents/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Ribonucleotides/pharmacology
EDAT- 2010/01/30 06:00
MHDA- 2010/04/09 06:00
CRDT- 2010/01/30 06:00
PHST- 2009/11/12 00:00 [accepted]
PHST- 2010/01/30 06:00 [entrez]
PHST- 2010/01/30 06:00 [pubmed]
PHST- 2010/04/09 06:00 [medline]
AID - 000276553 [pii]
AID - 10.1159/000276553 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2010;25(2-3):195-202. doi: 10.1159/000276553. Epub 2010 Jan 
      12.