PMID- 20110892
OWN - NLM
STAT- MEDLINE
DCOM- 20100402
LR  - 20211020
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 15
IP  - 1
DP  - 2010 Jan 13
TI  - Analysis of the main nucleosides in Cordyceps sinensis by LC/ESI-MS.
PG  - 305-14
LID - 10.3390/molecules15010305 [doi]
AB  - A sensitive, selective and reliable liquid chromatography-mass spectrometry 
      coupled with electrospray ionization interface method for simultaneous separation 
      and determination of thymine, adenine, adenosine and cordycepin in Cordyceps 
      sinensis has been established. The optimum separation for these analytes was 
      achieved using a gradient elution system and a 2.0 x 150 mm Shimadzu VP-ODS 
      column. 2-Chloroadenosine was used as internal standard for this assay. [M+H]+ 
      ions at m/z 127, 136, 268, 252 and 302 were chosen and selective ion monitoring 
      (SIM) mode was used for quantitative analysis of the four main nucleosides. The 
      regression equations were linear in the range of 1.0-117.5 microg x mL(-1) for 
      thymine, 1.8-127.0 microg x mL(-1) for adenine, 0.6-114.0 microg x mL(-1) for 
      adenosine and 0.5-107.5 microg x mL(-1) for cordycepin. The limits of 
      quantitation (LOQ) and detection (LOD) were 1.0 and 0.2 microg x mL(-1) for 
      thymine, 1.8 and 0.6 microg x mL(-1) for adenine, 0.6 and 0.1 microg x mL(-1) for 
      adenosine and 0.5 and 0.1 microg x mL(-1) for cordycepin, respectively. The 
      recoveries of the four nucleosides ranged from 98.47 to 99.32%. The developed 
      method was successfully used to determine nucleosides in Cordyceps sinensis from 
      different sources.
FAU - Xie, Jian-Wei
AU  - Xie JW
AD  - Department of Chemistry and Pharmaceutical, Quzhou College, Quzhou 324000, China.
FAU - Huang, Lan-Fang
AU  - Huang LF
FAU - Hu, Wei
AU  - Hu W
FAU - He, Yun-Biao
AU  - He YB
FAU - Wong, Kin Ping
AU  - Wong KP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100113
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Deoxyadenosines)
RN  - 0 (Nucleosides)
RN  - 0 (Solvents)
RN  - GZ8VF4M2J8 (cordycepin)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid/*methods
MH  - Cordyceps/*chemistry
MH  - Deoxyadenosines/analysis
MH  - Limit of Detection
MH  - Nucleosides/*analysis/chemistry
MH  - Reference Standards
MH  - Regression Analysis
MH  - Reproducibility of Results
MH  - Solvents/chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
PMC - PMC6257052
EDAT- 2010/01/30 06:00
MHDA- 2010/04/03 06:00
PMCR- 2010/01/13
CRDT- 2010/01/30 06:00
PHST- 2009/11/10 00:00 [received]
PHST- 2009/12/11 00:00 [revised]
PHST- 2010/01/07 00:00 [accepted]
PHST- 2010/01/30 06:00 [entrez]
PHST- 2010/01/30 06:00 [pubmed]
PHST- 2010/04/03 06:00 [medline]
PHST- 2010/01/13 00:00 [pmc-release]
AID - 15010305 [pii]
AID - molecules-15-00305 [pii]
AID - 10.3390/molecules15010305 [doi]
PST - epublish
SO  - Molecules. 2010 Jan 13;15(1):305-14. doi: 10.3390/molecules15010305.