PMID- 20112298
OWN - NLM
STAT- MEDLINE
DCOM- 20101123
LR  - 20151119
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 54
IP  - 7
DP  - 2010 Jul
TI  - A Maillard reaction product enhances eNOS activity in human endothelial cells.
PG  - 1031-8
LID - 10.1002/mnfr.200900330 [doi]
AB  - Nitric oxide (NO) produced by the endothelial nitric oxide synthase (eNOS) is an 
      important signaling molecule in the cardiovascular system. Although dietary 
      factors can modulate eNOS activity, putative effects of processed food are barely 
      investigated. We aimed to examine whether the model Maillard reaction product 
      3-hydroxy-2-methyl-1-propyl-4(1H)-pyridone (HMPP), formed from maltol or starch 
      and propylamine, affects the eNOS system. Incubation of EA.hy926 endothelial 
      cells with 30-300 microM HMPP for 18 h enhanced endothelial NO release measured 
      with the fluorescent probe diaminofluorescein-2 and eNOS activity determined by 
      the [14C]L-arginine-[14C]L-citrulline conversion assay. HMPP increased NO 
      production also in two different types of primary human endothelial cells. 
      Protein levels of eNOS and inducible NO synthase remained unaltered by HMPP. HMPP 
      inhibited eNOS activity within the first 2-4 h, whereas it potently increased 
      eNOS activity after 12-24 h. Levels of eNOS phosphorylation, expression of 
      heat-shock protein 90, caveolin-1 and various antioxidant enzymes were not 
      affected. Intracellular reactive oxygen species remained unchanged by HMPP. This 
      is the first study to demonstrate positive effects of a Maillard reaction product 
      on eNOS activity and endothelial NO production, which is considered favourable 
      for cardiovascular protection.
FAU - Schmitt, Christoph A
AU  - Schmitt CA
AD  - Department of Pharmacognosy, University of Vienna, Vienna, Austria.
FAU - Heiss, Elke H
AU  - Heiss EH
FAU - Schachner, Daniel
AU  - Schachner D
FAU - Aristei, Yasmin
AU  - Aristei Y
FAU - Severin, Theodor
AU  - Severin T
FAU - Dirsch, Verena M
AU  - Dirsch VM
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (4,5-diaminofluorescein)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Pyridones)
RN  - 30652-13-2 (1-propyl-2-methyl-3-hydroxypyrid-4-one)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - TPY09G7XIR (Fluorescein)
SB  - IM
MH  - Cardiovascular Diseases/prevention & control
MH  - Cell Line
MH  - Cells, Cultured
MH  - Computational Biology/methods
MH  - Endothelial Cells/*drug effects/*enzymology/metabolism
MH  - Expert Systems
MH  - Fluorescein/chemistry
MH  - Genes, Reporter
MH  - Humans
MH  - Indicators and Reagents/chemistry
MH  - Kinetics
MH  - *Maillard Reaction
MH  - Nitric Oxide/chemistry/metabolism
MH  - Nitric Oxide Synthase Type II/metabolism
MH  - Nitric Oxide Synthase Type III/antagonists & inhibitors/genetics/*metabolism
MH  - Organ Specificity
MH  - Osmolar Concentration
MH  - Promoter Regions, Genetic/drug effects
MH  - Pyridones/chemical synthesis/pharmacokinetics/*pharmacology
MH  - Up-Regulation/*drug effects
EDAT- 2010/01/30 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/01/30 06:00
PHST- 2010/01/30 06:00 [entrez]
PHST- 2010/01/30 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - 10.1002/mnfr.200900330 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2010 Jul;54(7):1031-8. doi: 10.1002/mnfr.200900330.