PMID- 20118379
OWN - NLM
STAT- MEDLINE
DCOM- 20100518
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 76
IP  - 6
DP  - 2010 Mar
TI  - Molecular monitoring and isolation of previously uncultured bacterial strains 
      from the sheep rumen.
PG  - 1887-94
LID - 10.1128/AEM.02606-09 [doi]
AB  - To estimate the contribution of uncultured bacterial groups to fiber degradation, 
      we attempted to retrieve both ecological and functional information on uncultured 
      groups in the rumen. Among previously reported uncultured bacteria, 
      fiber-associated groups U2 and U3, belonging to the low-GC Gram-positive 
      bacterial group, were targeted. PCR primers and fluorescence in situ 
      hybridization (FISH) probe targeting 16S rRNA genes or rRNA were designed and 
      used to monitor the distribution of targets. The population size of group U2 in 
      the rumen was as high as 1.87%, while that of group U3 was only 0.03%. Strong 
      fluorescence signals were observed from group U2 cells attached to plant fibers 
      in the rumen. These findings indicate the ecological significance of group U2 in 
      the rumen. We succeeded in enriching group U2 using rumen-incubated rice straw as 
      the inoculum followed by incubation in an appropriate medium with an agent 
      inhibitory for Gram-negative bacteria. Consequently, we successfully isolated two 
      strains, designated B76 and R-25, belonging to group U2. Both strains were 
      Gram-positive short rods or cocci that were 0.5 to 0.8 mum in size. Strain B76 
      possessed xylanase and alpha-l-arabinofuranosidase activity. In particular, the 
      xylanase activity of strain B76 was higher than that of xylanolytic Butyrivibrio 
      fibrisolvens H17c grown on cellobiose. Strain R-25 showed an 
      alpha-l-arabinofuranosidase activity higher than that of strain B76. These 
      results suggest that strains B76 and R-25 contribute to hemicellulose degradation 
      in the rumen.
FAU - Koike, S
AU  - Koike S
AD  - Research Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, 
      Sapporo, Hokkaido 060-8589, Japan. skoike7@anim.agr.hokudai.ac.jp
FAU - Handa, Y
AU  - Handa Y
FAU - Goto, H
AU  - Goto H
FAU - Sakai, K
AU  - Sakai K
FAU - Miyagawa, E
AU  - Miyagawa E
FAU - Matsui, H
AU  - Matsui H
FAU - Ito, S
AU  - Ito S
FAU - Kobayashi, Y
AU  - Kobayashi Y
LA  - eng
SI  - GENBANK/AB239489
SI  - GENBANK/AB521809
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100129
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.55 (alpha-N-arabinofuranosidase)
RN  - EC 3.2.1.8 (Endo-1,4-beta Xylanases)
SB  - IM
MH  - Animals
MH  - Bacterial Proteins/metabolism
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Endo-1,4-beta Xylanases/metabolism
MH  - Glycoside Hydrolases/metabolism
MH  - Gram-Positive Bacteria/*classification/enzymology/genetics/*isolation & 
      purification
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rumen/*microbiology
MH  - Sequence Analysis, DNA
MH  - Sheep/*microbiology
PMC - PMC2838016
EDAT- 2010/02/02 06:00
MHDA- 2010/05/19 06:00
PMCR- 2010/09/01
CRDT- 2010/02/02 06:00
PHST- 2010/02/02 06:00 [entrez]
PHST- 2010/02/02 06:00 [pubmed]
PHST- 2010/05/19 06:00 [medline]
PHST- 2010/09/01 00:00 [pmc-release]
AID - AEM.02606-09 [pii]
AID - 2606-09 [pii]
AID - 10.1128/AEM.02606-09 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2010 Mar;76(6):1887-94. doi: 10.1128/AEM.02606-09. Epub 
      2010 Jan 29.