PMID- 2012220
OWN - NLM
STAT- MEDLINE
DCOM- 19910509
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 260
IP  - 4 Pt 2
DP  - 1991 Apr
TI  - Opposing effects of diacetyl monoxime on contractility and calcium transients in 
      isolated myocardium.
PG  - H1153-9
AB  - Isolated, perfused guinea pig hearts were used to determine the effects of 
      diacetyl monoxime (DAM) on atrial rate, atrioventricular conduction time (AVCT), 
      and left ventricular peak systolic pressure (LVSP). Contractile force and calcium 
      transients were also monitored in isolated papillary muscles injected with 
      aequorin in the absence and presence of ryanodine (RYA). In the isolated heart, 
      0.2-5 mM DAM caused significant dose-dependent decreases in LVSP (up to 51%) 
      without change in atrial rate and AVCT. DAM (10 mM) produced a small slowing of 
      atrial rate, no change in AVCT, and a 76% decrease in LVSP; 20 and 30 mM DAM 
      further reduced LVSP, decreased atrial rate 38%, and produced AV dissociation. In 
      isolated, paced papillary muscles, 2, 10, and 30 mM DAM decreased contractile 
      force 27, 58, and 87%, respectively, while calcium transients increased by -9, 
      38, and 225%, respectively. All cardiac effects of DAM were readily reversible. 
      RYA (1 microM) alone and with DAM (30 mM) decreased contractile force by 30 and 
      99%, respectively and decreased calcium transients by 59 and 74%, respectively. 
      This study shows that low concentrations of DAM have little effect on electrical 
      activity but greatly depress contractility and modify intracellular handling of 
      calcium.
FAU - Marijic, J
AU  - Marijic J
AD  - Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226.
FAU - Buljubasic, N
AU  - Buljubasic N
FAU - Stowe, D F
AU  - Stowe DF
FAU - Turner, L A
AU  - Turner LA
FAU - Kampine, J P
AU  - Kampine JP
FAU - Bosnjak, Z J
AU  - Bosnjak ZJ
LA  - eng
GR  - HL-0901/HL/NHLBI NIH HHS/United States
GR  - HL-34708/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 15662-33-6 (Ryanodine)
RN  - 19SQ93LM6H (diacetylmonoxime)
RN  - K324J5K4HM (Diacetyl)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Atrial Function
MH  - Blood Pressure
MH  - Calcium/*metabolism
MH  - Diacetyl/*analogs & derivatives/pharmacology
MH  - Guinea Pigs
MH  - Heart Atria/drug effects
MH  - Heart Conduction System/drug effects/physiology
MH  - Heart Rate/drug effects
MH  - Heart Ventricles/drug effects
MH  - Myocardial Contraction/*drug effects
MH  - Myocardium/*metabolism
MH  - Papillary Muscles/drug effects/physiology
MH  - Pressure
MH  - Ryanodine/pharmacology
MH  - Ventricular Function
EDAT- 1991/04/01 00:00
MHDA- 1991/04/01 00:01
CRDT- 1991/04/01 00:00
PHST- 1991/04/01 00:00 [pubmed]
PHST- 1991/04/01 00:01 [medline]
PHST- 1991/04/01 00:00 [entrez]
AID - 10.1152/ajpheart.1991.260.4.H1153 [doi]
PST - ppublish
SO  - Am J Physiol. 1991 Apr;260(4 Pt 2):H1153-9. doi: 
      10.1152/ajpheart.1991.260.4.H1153.