PMID- 20124217
OWN - NLM
STAT- MEDLINE
DCOM- 20100430
LR  - 20210206
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 115
IP  - 15
DP  - 2010 Apr 15
TI  - Relapse of leukemia with loss of mismatched HLA resulting from uniparental disomy 
      after haploidentical hematopoietic stem cell transplantation.
PG  - 3158-61
LID - 10.1182/blood-2009-11-254284 [doi]
AB  - We investigated human leukocyte antigen (HLA) expression on leukemic cells 
      derived from patients at diagnosis and relapse after hematopoietic stem cell 
      transplantation (HSCT) using flow cytometry with locus-specific antibodies. Two 
      of 3 patients who relapsed after HLA-haploidentical HSCT demonstrated loss of HLA 
      alleles in leukemic cells at relapse; on the other hand, no loss of HLA alleles 
      was seen in 6 patients who relapsed after HLA-identical HSCT. Single-nucleotide 
      polymorphism array analyses of sorted leukemic cells further revealed the copy 
      number-neutral loss of heterozygosity, namely, acquired uniparental disomy on the 
      short arm of chromosome 6, resulting in the total loss of the mismatched HLA 
      haplotype. These results suggest that the escape from immunosurveillance by the 
      loss of mismatched HLA alleles may be a crucial mechanism of relapse after 
      HLA-haploidentical HSCT. Accordingly, the status of mismatched HLA on relapsed 
      leukemic cells should be checked before donor lymphocyte infusion.
FAU - Villalobos, Itzel Bustos
AU  - Villalobos IB
AD  - Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, 
      466-8550, Japan.
FAU - Takahashi, Yoshiyuki
AU  - Takahashi Y
FAU - Akatsuka, Yoshiki
AU  - Akatsuka Y
FAU - Muramatsu, Hideki
AU  - Muramatsu H
FAU - Nishio, Nobuhiro
AU  - Nishio N
FAU - Hama, Asahito
AU  - Hama A
FAU - Yagasaki, Hiroshi
AU  - Yagasaki H
FAU - Saji, Hiroh
AU  - Saji H
FAU - Kato, Motohiro
AU  - Kato M
FAU - Ogawa, Seishi
AU  - Ogawa S
FAU - Kojima, Seiji
AU  - Kojima S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100201
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantigens)
SB  - IM
MH  - Blast Crisis/genetics/immunology
MH  - Child
MH  - Chromosomes, Human, Pair 6/genetics
MH  - Cytotoxicity, Immunologic
MH  - HLA Antigens/*immunology
MH  - *Haploidy
MH  - *Hematopoietic Stem Cell Transplantation
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Isoantigens/immunology
MH  - Leukemia/*immunology
MH  - Recurrence
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Tissue Donors
MH  - Uniparental Disomy/*genetics
EDAT- 2010/02/04 06:00
MHDA- 2010/05/01 06:00
CRDT- 2010/02/04 06:00
PHST- 2010/02/04 06:00 [entrez]
PHST- 2010/02/04 06:00 [pubmed]
PHST- 2010/05/01 06:00 [medline]
AID - S0006-4971(20)56504-X [pii]
AID - 10.1182/blood-2009-11-254284 [doi]
PST - ppublish
SO  - Blood. 2010 Apr 15;115(15):3158-61. doi: 10.1182/blood-2009-11-254284. Epub 2010 
      Feb 1.